怡瑞胶囊对高脂血症大鼠肝脏中嘌呤受体P_2X_7,A_2A表达的影响  

Effect of Yirui Capsule on Expression of Purinergic Receptors P_2X_7 and A_2A in Livers of Hyperlipidemia Rats

作　　者：周海松[1] 王青[1] 易浪[1] 李文治 罗其昌 董燕[1] 王培训[1] 

机构地区：[1]广州中医药大学临床药理研究所,广州510405 [2]无限极(中国)有限公司,广东江门529156

出　　处：《中国实验方剂学杂志》2015年第19期142-145,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨高脂血症大鼠炎症水平与肝组织中嘌呤受体P2X7,A2A表达的关系及怡瑞胶囊的干预作用。方法:SD雄性大鼠50只,随机分为正常组10只,喂饲维持饲料;高脂饲料组40只,喂饲高脂饲料,14 d后再分为模型组和怡瑞胶囊低、中、高剂量组(140,280,560 mg·kg-1),每组10只。ig给药30 d,采血分离血清,检测血脂水平。酶联免疫吸附试验(ELISA)检测白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的水平,反转录PCR技术(RT-PCR)检测肝组织的嘌呤受体P2X7,A2A的表达。结果:与正常组比较,高脂血症模型组大鼠血清中炎症因子IL-1β和TNF-α含量显著升高(P<0.01),肝脏中嘌呤受体P2X7,A2A的表达水平升高(P<0.01),与模型组比较,怡瑞胶囊各剂量组IL-1β和TNF-α的水平显著降低(P<0.01),肝组织中P2X7,A2A的表达水平显著降低(P<0.01)。结论:怡瑞胶囊调节高脂血症大鼠肝组织中嘌呤受体的表达可能与其降低炎症水平和改善脂代谢有关。Objective： To study the relationship between the inflammation and expression of hepatic purinergic receptors P2X7 and A2A in hyperlipidemia rats, and the intervention effect of Yirui capsule. Method： Fifty male rats were randomly divided into two groups. Ten rats were fed with normal diets as normal group, and forty rats were fed with high-fat diets as the observation group. After 14 d, the forty rats were further randomly divided into model group and Yirui capsule low, middle, high dose groups, 10 rats for each group. Yirui capsules were administered ig to the treatment group for 30 days at 140, 280, 560 mg .kg-1 respectively. And then, the levels of lipids in serum were detected, tumor necrosis factor-α （TNF-α） and interleukin-1β（IL-1β） in serum were detected by enzyme-linked immunosorbant assay （ELISA） , and the expression of purinergic receptors P2X7 and A2A in livers were analyzed by RT-PCR. Result： The content of inflammatory cytokines TNF-α, IL-1β and the expression of purinergic receptors P2X7 and A2A in livers were increased in model group, compared with normal group （P 〈 0.01 ）. The content of inflammatory cytokines TNF-α, IL-1β and the expression of purinergic receptors P2X7 and A2A in livers were decreased in Yirui treatment group, compared with model group （P 〈 0.01 ）. Conclusion： Yirui capsule can regulate the expression of purinergic receptors in rat＇s liver, which is possibly relevant to the anti-inflammatory properties and amendment of lipid metabolism.

关 键 词：高脂血症 炎症 嘌呤受体 怡瑞胶囊 

分 类 号：R285.5[医药卫生—中药学]