肺炎链球菌荚膜多糖分子大小及分子量分析方法的比较  

RESEARCH ON THE EXPLICIT ALGORITHM OF THE ROLL FLATTENING RADIUS IN HOT CONTINUOUS ROLLING

作　　者：韦丽君[1] 尹晓鹏[1] 孙宪萍[1] 孙广琪 

机构地区：[1]长春工业大学机电工程学院,长春130012 [2]甘肃宏达铝业公司,兰州730060

出　　处：《机械强度》2015年第5期947-951,共5页Journal of Mechanical Strength

摘　　要：目的 3种方法分析10个血清型肺炎链球菌荚膜多糖（pneumococcal capsular polysaccharides,Pn Ps）样品的分子大小及分子量,并对这3种方法进行比较。方法采用Sepharose CL-4B凝胶排阻层析（SEC）技术检测1型、2型、4型、8型、9N型、12F型、14型、18C型、19A型和23F型Pn Ps各2~3批次样品在色谱柱中的分配系数（KD）和样品组分特征;采用高效凝胶排阻色谱-示差折光检测器（high performance size-exclusion chromatography with refractive index,HPSEC-RI）法建立Pullulan标准品分子量标准曲线,计算各Pn Ps样品的重均分子量（weight-average molecular weight,Mw）;采用高效凝胶排阻色谱-多角度激光光散射仪/示差折光检测器联用技术（high performance size-exclu-sion chromatography with multi-angle laser light scattering and refractive index,HPSEC-MALLS/RI）检测各Pn Ps样品的Mw、数均分子量（number-average molecular weight,Mn）、多分散水平（Mw/Mn）及均方根旋转半径（root mean square ratio of gyration,Rg）;对HPSEC-RI法和HPSEC-MALLS/RI法检测Pullulan分子量标准品的结果进行比较。结果 SEC技术不同化学方法检测的同批次Pn Ps样品的KD值十分接近,且主峰形态也比较近似,个别批次Pn Ps不同化学方法获得的峰形有差别。经HPSEC-RI检测Pullulan分子量标准品获得的回归方程为y=-0.278 x＋10.13,r2=0.998,各Pn Ps样品的分子量为1.812×105~1.246×106。HPSEC-MALLS/RI联用技术检测的各批Pn Ps样品的Mw分布于9.898×105~1.471×105,Rg为84.9~25.4 nm,同一血清型不同批次样品Rg间的大小关系与其Mw间的大小关系具有较好一致性。结论结合多种方法检测多糖样品的分子大小和分子量,可更客观、全面地了解Pn Ps分子的各种性质。In order to enhance the computational accuracy of the roll flattening radius of the hot strip mills, the explicit algorithm of the roll flattening radius was put forward. Compared with the traditional Hitchcock equation, the iteration is omitted in the calculation process. The computational accuracy of the roll flattening radius automatic control model can be enhanced in hot strip roiling. Meanwhile, the response speed of the roll flattening radius control system can be improved too. Taken the 500mm strip 9 hot continuous rolling strip mills for example, the roll flattening radius is calculated. The computational accuracy can enhance 2. 14% at most.

关 键 词：肺炎链球菌荚膜多糖 分子大小 分子量 

分 类 号：TG331[金属学及工艺—金属压力加工]