人参15个组织器官中皂苷量与6种人参皂苷生物合成基因表达的相关性研究  

Correlation of gene expression related to amount of ginseng saponin in 15 tissues and 6 kinds of ginseng saponin biosynthesis

作　　者：王康宇[1] 张美萍[1] 李闯[1] 蒋世翠[1] 尹锐[1] 孙春玉[1] 王义[1] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118

出　　处：《中国中药杂志》2015年第16期3168-3173,共6页China Journal of Chinese Materia Medica

基　　金：国家高技术研究发展计划(863)项目(2013AA102604-3)

摘　　要：以四年生红果期吉林人参的15个组织器官为材料,利用HPLC和香草醛-硫酸比色法分别测定15个组织器官中6种单体皂苷(人参皂苷Rg1,Re,Rb1,Rc,Rb2,Rd)和总皂苷含量;同时,利用实时荧光定量PCR测定15个组织器官中法尼基焦磷酸合成酶(farnesyl pyrophosphate synthase,FPS)、鲨烯合成酶(squalene synthase,SQS)、鲨烯环氧酶(squalene epoxidase,SQE)、氧化鲨烯环化酶(oxidized squalene cyclase,OSC)、β-香树脂合成酶(β-amyrin synthase,β-AS)和细胞色素P450超家族(cytochrome P450 family,P450)基因的相对表达量。研究试图通过相关性分析,获得吉林人参15个组织器官中人参皂苷含量与生物合成途径相关酶基因表达量之间相关关系。结果表明,6种单体皂苷含量之间具有线性正相关的协同增减趋势,而单体皂苷含量与总皂苷含量之间具有相关性极显著(P<0.01)的正相关关系;总皂苷含量与人参中SQS,OSC,β-AS,SQE,P450,FPS基因之间的相关性极显著(P<0.01),单体皂苷含量与6种酶基因之间相关性各不相同。说明这6种参与人参皂苷生物合成酶基因调控人参总皂苷与单体皂苷的生物合成,它们在人参不同组织器官中的表达呈现协同增减趋势,并以集群协调表达的方式来调控人参皂苷生物合成。Fifteen tissues of 4-year-old fruit repining stage Jilin ginseng were chosen as materials, six kinds of monomer saponins （ginsenosides Rg1, Re, Rb1, Re, Rb2 and Rd） content in 15 tissues was measured by HPLC and vanillin-sulfuric acid method. The relative expression of FPS, SQS, SQE, OSC, 13-AS and P450 genes in 15 tissues was analyzed by real-time PCR. The correlations be- tween ginseng saponin content in 15 tissues of Jilin ginseng and biosynthetic pathway -related genes were obtained. The results showed that was a synergistic increase and decrease trend of positive linear correlation among six kinds of monomer saponin content, and there was a significantly （ P 〈 0. 01 ） positive correlation between monomer saponin content and total saponins content. Monomer saponin con- tent and 6 kinds of enzyme gene correlation were different. Biosynthesis of ginseng total saponins and monomer saponin were regulated by six kinds of participation ginsenoside biosynthesis enzyme genes, the expression of these six kinds of genes in different tissues of gin- seng showed collaborative increase and decrease trend, and regulated biosynthesis of ginseng ginsenoside by group coordinative manner.

关 键 词：人参 人参皂苷 基因表达 相关性 人参皂苷生物合成 

分 类 号：S567.51[农业科学—中草药栽培]