石蒜碱诱导肺癌A549细胞凋亡作用研究  

Study on effect of lycorine in inducing apoptosis of pulmonary carcinoma cell A549

作　　者：张炜[1] 崔恩海[1] 

机构地区：[1]湖州市中心医院呼吸内科,浙江湖州313001

出　　处：《中国中药杂志》2015年第16期3278-3282,共5页China Journal of Chinese Materia Medica

摘　　要：为探讨石蒜碱诱导肺癌A549细胞的凋亡作用及其机制。该研究以肺癌A549细胞为实验对象,通过不同浓度的石蒜碱(0,0.5,1.0,2.0,4.0,8.0μmol·L-1)处理A549细胞,MTT观察细胞增殖情况;Annexin V-FITC/PI双标法检测细胞的凋亡率;酶标仪检测凋亡因子Bcl-2,Bax,p53的活性;流式细胞仪检测细胞线粒体膜电位变化;Real-time PCR检测凋亡相关基因Bcl-2,Bax,p53和Survivin的表达。研究结果表明:石蒜碱可显著抑制A549细胞增殖(P<0.05)、诱导A549细胞凋亡(P<0.05)。同时增强Bax和p53的活性,降低Bcl-2活性和线粒体膜电位,显著改变Bcl-2,Bax,p53和Survivin的基因表达(P<0.05)。综上表明石蒜碱可诱导A549细胞凋亡,具有治疗肺癌的应用前景,其机制可能与其调节Bcl-2信号通路中相关因子活性有关。To explore the effect of lycorine in inducing apoptosis of pulmonary carcinoma cell A549 and its mechanism. In the study, pulmonary carcinoma cell A549 were taken as the experimental subject and processed with different concentrations of lycorine （0, 0. 5, 1. 0, 2. 0, 4. 0 and 8.0μmol . L-1 ）. The MTT method was used to observe the cell proliferation. The apoptosis rate of A549 cells was determined by Annexin FITC/PI double staining. The microplate reader was used to detect the activities of Bcl-2, Bax and p53. The changes in mitochondrial membrane potential were measured by the flow cytometry. The expressions of apoptosis-related factors Bcl-2, Bax, p53 and Survivin were determined by Real-time PCR. The results showed that lycorine significantly inhibited the proliferation of A549 cells （ P 〈 0. 05 ） , induced the apoptosis on A549 cells （ P 〈 0. 05 ）, increased the activities of Bax and p53, re- duced Bcl-2 activity and mitochondrial membrane potential, and notably changed the gene expressions of Bcl-2, Bax, p53 and Survivin （P 〈 0. 05 ）. In conclusion, lycorine can induce the apoptosis of A549 cells and be applied to treat pulmonary carcinoma. Its mecha- nism may be related to the activation of relevant factors in Bcl-2 signaling pathway.

关 键 词：石蒜碱 肺癌A549细胞 凋亡 信号通路 

分 类 号：R285[医药卫生—中药学]