红花(Carthamus tinctorius L.)不同开花时期的转录组测序及黄酮合成相关基因的验证  

Analysis of Genes Involved in Saffl ower Flavonoids Biosynthesis Based on Saffl ower Transcriptome in Different Flowering

作　　者：刘秀明[1,2] 张宇[2] 姚娜[1] 崔喜燕[1] 王立勇[1] 董园园[1] 王南[1] 李晓薇[1,2] 李海燕[1,2] 李校堃[1] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,长春130118 [2]吉林农业大学生命科学学院,长春130118

出　　处：《中国细胞生物学学报》2015年第9期1207-1215,共9页Chinese Journal of Cell Biology

基　　金：国家高技术研究发展计划(863计划)(批准号:2011AA100606);国家自然科学基金(批准号:31101172;31201237)资助的课题~~

摘　　要：应用454测序技术对红花(Carthamus tinctorius L.)初花期和盛花期两个样本进行转录组测序,分别获得了577 664和562 930条Clean reads片段,其平均长度分别为427 bp和436 bp。差异表达分析结果发现,在初花期获得40 139个基因,盛花期获得39 768个基因,在两个时期同时表达的基因有28 316个。COG/KOG分析中,获得了大量与代谢相关的功能注释信息,注释到比例最高的为功能预测,占24.681%。KEGG Pathway富集分析中,找到红花黄酮化合物合成途径中的关键酶基因,利用Real-time PCR方法验证了三个基因的正确性。表达量分析表明,查尔酮合酶在两个红花品种中各个开花时期的相对表达量最高。454 sequencing technology was applied to sequencing transcriptome of safflower samples of early flowering and full-bloom stage, respectively. We obained 577 664 and 562 930 Clean reads fragments, with average length 427 bp and 436 bp, respectively. 40 139 genes were expressed in early flowering in transcriptome. 39 768 genes were expressed in full-bloom flowering through differential expression analysis. 28 316 genes were expressed simultaneously in these two samples. Massive annotation information related to metabolic was obtained through COG/KOG analysis. Among them, 24.681% was highest in annotating as basic functionality. Safflower flavonoids metabolic pathway was found and verified by Real-time PCR in KEGG Pathway analysis. Quantitative PCR analysis showed that the CHS had the highest expression level in the flowering period of two safflower var.

关 键 词：红花 转录组测序 黄酮合成 

分 类 号：S567.219[农业科学—中草药栽培]