莪术油诱导人胃腺癌SGC-7901细胞凋亡的研究  

Research of Zedoary Turmeric Oil Induced Apoptosis of Human Gastric Carcinoma SGC-7901 Cells

作　　者：李玲玲[1] 邵淑丽[1] 孙宏岩[1] 张伟伟[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006

出　　处：《中国细胞生物学学报》2015年第9期1235-1241,共7页Chinese Journal of Cell Biology

基　　金：黑龙江省自然科学基金项目(批准号:C201241);黑龙江省教育厅科学技术项目(批准号:12511611)资助的课题~~

摘　　要：该实验目的是探究莪术油(zedoray turmeric oil,ZTO)诱导人胃腺癌SGC-7901细胞凋亡的作用。不同浓度的ZTO作用人胃腺癌SGC-7901细胞48 h后,采用台盼兰拒染法检测细胞生长抑制率;光学显微镜、荧光显微镜、透射电镜下观察细胞的形态和超微结构;琼脂糖凝胶电泳检测DNA片段化;Annexin V-FITC法检测细胞的凋亡率;实时荧光定量PCR和Western blot检测Bax、Bcl-2 m RNA和蛋白表达水平。结果显示,作用人胃腺癌SGC-7901细胞48 h,ZTO的最佳浓度是110μg/m L,IC50为(108.002±0.305)μg/m L;显微镜下细胞呈不同程度的凋亡特征;DNA电泳可见梯状条带;最佳药物浓度作用细胞48 h的早期凋亡率为(25.07±0.82)%;Bax表达水平升高,Bcl-2表达水平降低,Bax/Bcl-2比值显著升高(P<0.05),表明ZTO通过上调Bax表达、下调Bcl-2表达诱导人胃腺癌SGC-7901细胞凋亡。The study of this experiment was to explore the effects for the apoptosis trigged by zedoary turmeric oil （ZTO） on the human gastric carcinoma SGC-7901 cells. The effects of ZTO were studied by trypan blue staining method, light microscope, fluorescence microscope, transmission electron microscope, flow cytometry, Real-time PCR and Western blot. After the human gastric carcinoma SGC-7901 cells treated with various concentrations of ZTO for 48 h, the growth inhibition rate of cell was determined by trypan blue staining method. The morphology and ultrastructure were observed by light microscope, fluorescence microscope and transmission electron microscope. DNA fragmentation was assayed by agarose gel electrophoresis. The apoptotic rate was investigated by flow cytometry. The expression levels of Bax and Bcl-2 were explored by Real-time PCR and Western blot. The results showed that the concentration for the treatment was optimized to 110 ktmol/L in this study, and the ICs0 value was （108.002±0.305）μg/mL. The early apoptotic rate was reached to （25.07±0.82）% under the microscope. The typical DNA ladder was showed on agarose gel electrophoresis. Importantly, the expression level Bax increased and the expression level of Bcl-2 decreased, so that the ratio of Bax and Bcl-2 increased significantly （P〈0.05）. These results suggested that ZTO could induce the apoptosis of the human gastric carcinoma SGC-7901cells by up-regulating the expression of Bax and down-regulating the expression of Bcl-2.

关 键 词：莪术油 胃腺癌SGC-7901 凋亡 

分 类 号：R285[医药卫生—中药学]