小半夏加茯苓汤对H22荷瘤小鼠瘤体中颗粒酶B和穿孔素表达的影响  

The Effect on the Expression of Perforin and Granzyme B from H22 Tumor-burdened Mice Intervened by Xiao Ban Xia Plus Fuling Decoction

作　　者：韦佳[1] 杨长福[1] 陈倩[1] 冯泳[1] 何前松[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《时珍国医国药》2015年第9期2086-2089,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81160425/H2705)

摘　　要：目的观察小半夏加茯苓汤对H22荷瘤小鼠瘤体中的颗粒酶B及穿孔素表达的影响。方法建立体内H22荷瘤小鼠模型,随机分为荷瘤模型组、小半夏加茯苓汤高剂量组、小半夏加茯苓汤中剂量组、小半夏加茯苓汤低剂量组、顺铂组,每组24只。接种次日开始给药,荷瘤模型组给予生理盐水10g·kg-1,1次/d;小半夏加茯苓汤高、中、低剂量组,分别给予含4g、2g、1g生药·ml-1小半夏加茯苓汤水煎剂40,20,10g·kg-1,1次/d;顺铂组腹腔注射0.9%氯化钠溶液稀释顺铂5mg·kg-1,分别于第1,4,7,10天各给药1次,实验第11日称重断髓处死小鼠,取瘤体。采用逆转录-聚合酶链反应(RT-PCR)和免疫组化(IHC)检测瘤组织中的颗粒酶B和穿孔素的表达量情况。结果与荷瘤模型组比较,小半夏加茯苓汤高剂量组体重明显升高(P<0.05),小半夏加茯苓汤高、中剂量组瘤重明显降低(P<0.05),顺铂组瘤重更低(P<0.01);小半夏加茯苓汤高、中剂量组,顺铂组颗粒酶B、穿孔素基因表达量升高;各给药组颗粒酶B与穿孔素蛋白表达均显著升高(P<0.01)。与顺铂组比较,各组小半夏加茯苓汤体重、瘤重显著升高(P<0.01)。结论小半夏加茯苓汤升高H22荷瘤小鼠瘤体中的颗粒酶B及穿孔素表达量,这可能是诱导细胞凋亡的机制之一。Objective To observe the effect on perforin and granzyme B＇s expression of H22 liver tumor - burdened mice intervened by Xiao Ban Xia Plus Fuling Decoction. Methods The model of H22 liver tumor - burdened mice was established. The mice were divided into five groups, including model group, Xiao Ban Xia Plus Fuling Decoction high group, middle group, low group and cis - platinum group, each group 24 mice. Medicine was given from the day after inoculating once a day. Model group was fed normal saline 10g · kg^-1 ;Xiao Ban Xia Plus Fuling Decoction groups made by 4g,2g and lg crude drugs · mL^- 1 decoction were given a gavage of 40,20,10g · kg^-1 ;eis - platinum group was given intraperitoneal injection 5mg· kg^-1 diluted by 0.9% sodium chloride solution,on the 1st day,4th day,Tth day and 10th day,once a day. Mice were sacrificed on the llth day,and the tumor were obtained. Apply Reverse Transcription -Polymerase Chain Reaction（ RT- PCR） and Immunohistochemistry（IHC） to detect the expression of perforin and granzyme B from mice tumor. Results Compared with the model group, the weight of Xiao Ban Xia Plus Fuling Decoction high group is improved significantly （ P 〈 0.05 ） ; the tumor weight of Xiao Ban Xia Plus Fuling Decoction high group and middle group is reduced obviously （P 〈 0.05 ）, and cis - platinum group is lower （P 〈 0.01 ） ; the expression of granzyme B and perforin is improved fed by Xiao Ban Xia Plus Fuling Decoction high, middle dose and cis - platinum ; the protein expression of granzyme B and perforin in every group is improved significantly（ P 〈 0.01 ）. Compared with cis -platinum group, mice weight and tumor weight of other groups are higher（P 〈 0.01 ）. Conclusion Xiao Ban Xia Plus Fuling Decoction can improve the expression of granzyme B and perforin of tumor from H22 liver tumor - burdened mice, whose mechanism is probably related to inducing cell apoptosis.

关 键 词：小半夏加茯苓汤 H22荷瘤小鼠 颗粒酶B 穿孔素 

分 类 号：R285.5[医药卫生—中药学]