三子养亲汤中白芥子-莱菔子合并提取工艺的正交试验优化  

Optimization of Merger Extracting Technology for Mustard Seed-Radish Seed of SanZiYangQinTang by Orthogonal Test

作　　者：谢清春[1] 吕竹芬[1] 林世源[1] 陈燕忠[1] 

机构地区：[1]广东药学院药物研究所/广东省药物新剂型重点实验室,广东广州510006

出　　处：《时珍国医国药》2015年第9期2164-2166,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广东省自然科学基金(No.S2011010002485)

摘　　要：目的优选白芥子-莱菔子合并提取的最佳工艺。方法采用高效液相色谱法测定两味药材中的芥子碱含量,以其作为考察指标,通过正交试验设计优化白芥子-莱菔子合并提取工艺。结果两味药材合并提取的最佳工艺为脱脂后,加入10倍量70%乙醇于85℃水浴加热回流提取3次,每次1 h。结论该提取工艺下提取率较高,操作稳定可行。Objective To optimize the merger extracting technology for Mustard Seed - Radish Seed. Methods The orthngonal test was designed on optimizing the merger extracting process with the content of mustard alkali as the index which was measured by high performance liquid chromatography. Results The proper extraction process of the two herbs was extracted with 10 times a- mount of 70% ethanol for 3 times, heated refluxed 1 hour each time. Conclusion The extraction process with high yield was sta- ble and feasible.

关 键 词：正交试验设计 合并提取 白芥子 莱菔子 

分 类 号：R284.2[医药卫生—中药学]