清开灵注射液体对H1N1、H5N1和H7N9流感病毒的作用研究  

Anti-viral Effects of Qingkailing Injection Against Influenza H1N1,H5N1,and H7N9 Virus in Vitro

作　　者：赵利华[1] 陈全姣[1] 

机构地区：[1]中国科学院武汉病毒研究所,湖北武汉430071

出　　处：《中药新药与临床药理》2015年第5期644-648,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：中国科学院流感研究与预警中心项目(Y505031YZ1)

摘　　要：目的研究清开灵注射液体外抗H1N1、H5N1、H7N9流感病毒的作用。方法以利巴韦林注射液作为阳性对照药物,通过测定细胞培养液上清血凝滴度(HA)和半数组织培养感染剂量(TCID50),评价清开灵注射液不同给药方式对H1N1、H5N1和H7N9流感病毒的抑制作用,并且通过MTT法测定清开灵注射液不同给药方式体外抗H1N1流感病毒作用。结果清开灵注射液半数中毒浓度(TC50)为62.5μL·m L-1;清开灵注射液有明显抑制流感病毒增殖的作用,以中和、治疗、预防的给药方式能显著降低H1N1、H5N1流感病毒的HA和TCID50,且以中和与治疗的给药方式作用最强,以治疗的给药方式能显著降低H7N9流感病毒的HA和TCID50;清开灵注射液以中和与治疗的给药方式体外抑制H1N1流感病毒的半数有效浓度(EC50)分别为4.07μL·m L-1和2.90μL·m L-1,治疗指数(TI)分别为15.36、21.55。结论清开灵注射液具有明显的体外抗H1N1、H5N1、H7N9流感病毒作用。Objective To study the anti-viral effects of influenza virus in vitro. Methods Using Ribavirin injection Qingkailing injection against H1N1, H5N1, as the positive control drug, hemagglutination and H7N9 （HA） and median tissue culture infectious dose（TCIDso） of cell culture supernatant were determined to assess the inhibitory effect of Qingkailing injection for different medication purposes on H1N1, H5N1, H7N9 influenza virus. The anti-H1N1 influenza virus effect of Qingkailing Injection by different application methods was measured by MTT assay. Results The median toxic concentration（TCso） of Qingkailing injection was 62.5 μL·mL-1. In vitro, Qingkailing injection used for the purpose of neutralization, treatment, and prevention could significantly inhibit the virus proliferation, reduce the HA and TCID50 of H1N1 and H5N1 influenza virus, and the effects of Qingkailing injection used for the purpose of neutralization and treatment were stronger. By the medication method for treatment, Qingkailing injection could significantly reduce the HA and TCIDs0 of H7N9 influenza virus. The median effective concentration（ECs0） in vitro of Qingkailing injection by neutralization and treatment administration way was 4.07, 2.90 μL·mL-l, and therapeutic index was 15.36, 21.55 respectively. Conclusion Qingkailing injection has significant anti-H1N1, H5NI, H7N9 influenza virus effect in vitro.

关 键 词：清开灵注射液 流感病毒 抗病毒作用 

分 类 号：R285.5[医药卫生—中药学]