鼻吸入薄荷油对小鼠精神疲劳行为及脑内氨基酸类神经递质的影响  

Effects of Nasal Inhalation of Peppermint Oil on Mental Fatigue Behaviors and Amino Acid Neurotransmitters in Mice

作　　者：梁浩明[1] 龙晓英[1] 卢耀文[1] 宋浩辉 叶静宜[1] 

机构地区：[1]广东药学院,广东广州510006

出　　处：《中药新药与临床药理》2015年第5期649-654,共6页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的研究鼻吸入薄荷油对小鼠精神疲劳行为及脑内氨基酸类神经递质的影响。方法雄性KM小鼠随机分为空白对照组、蒸馏水对照组、睡眠剥夺模型组、薄荷油组,采用强迫游泳实验和戊巴比妥钠诱导睡眠实验观察小鼠情绪与睡眠行为的改变;水上站立法对小鼠进行连续48 h睡眠剥夺,旷场实验观察自发活动的变化,高效液相柱前衍生2,4-二硝基氟苯法测定给药后2 h内嗅球、下丘脑和皮质中天冬氨酸(Asp)、谷氨酸(Glu)、甘氨酸(Gly)、γ-氨基丁酸(GABA)含量。结果与空白对照组比较,鼻吸入薄荷油后,小鼠的强迫游泳不动时间和戊巴比妥钠诱导的睡眠时间均显著缩短。与睡眠剥夺模型组比较,鼻吸入薄荷油能极显著地提高睡眠剥夺小鼠的自发活动;嗅球和下丘脑Asp含量在10 min后显著提升,30 min后开始回落;各脑区Gly含量则无显著性改变;三个脑区Glu/GABA比值有显著性升高,15 min时达到峰值后逐渐回落至睡眠剥夺模型组水平。给药后2 h内的相关性分析表明,嗅球分别与下丘脑、皮层的Asp和Glu存在较强相关性。结论鼻吸入薄荷油可改善精神疲劳症状,具有良好的抗精神疲劳作用,其作用机制与其通过嗅觉系统调控精神疲劳相关脑区氨基酸类神经递质含量有关。Objective To investigate the influence of nasal inhalation of peppermint oil on mental fatigue behaviors and amino acid neurotransmitters in mice. Methods Male KM mice were divided into blank control group, distilled water control group, sleep deprived model group and peppermint oil group. Forced swimming test and pentobarbital sodium induced sleeping test were used to observe the changes in emotion and sleep of mice. After 48-hour sleep deprivation by the method of water standing, mice were subjected to open field test for the observation of spontaneous activity of the mice. The levels of amino acid neurotransmitters of aspartate （Asp）, glutamic acid （Glu）, glycine （Gly）, gamma aminobutyric acid（GABA） in the olfactory bulb, hypothalamus and cortex of the sleep-deprived mice were detected by precolumn derivative dinitrofluorobenzene HPLC （RP-DNFB-HPLC） within 2 h after medication of peppermint oil. Results Compared to the blank control group, the immobility time in forced swimming test and the sleeping time in pentobarbital sodium induced sleeping test were significantly shortened after the treatment with peppermint oil. Compared to the sleep deprived model group, the spontaneous activity of sleep deprived mice in peppermint oil group was dramatically increased, Asp content in the olfactory bulb and hypothalamus was promoted remarkably in 10 min and began to fall in 30 min, Gly stayed unchanged while Glu/GABA was elevated dramatically in the three cerebral regions and then went down after a peak at 15 min. The results of correlation analysis showed that Asp and Glu contents in the olfactory bulb were markedly correlated with those in the Conclusion Nasal inhalation of peppermint oil has anti-mental hypothalamus and cortex within 2 hours after treatment. fatigue effect on mice, and its mechanism may depend on the regulation of amino acid neurotransmitters in the cerebral regions related to mental fatigue through olfactory system.

关 键 词：薄荷油 睡眠剥夺 行为学 氨基酸类神经递质 嗅觉系统 

分 类 号：R285.5[医药卫生—中药学]