顶空气相色谱法测定白芍甘草提取物中有机溶剂残留量  

Determination of Organic Solvent Residuals in Extract of Radix Paeoniae Alba and Radix Glycyrrhizae by Capillary Gas Chromatography with Headspace Sampling

作　　者：江斌[1] 何伟[1] 李勇[1] 吉国辉[1] 林伟鑫[1] 朱秀辉 

机构地区：[1]广东药学院药物研究所/广东省药物新剂型重点实验室,广东广州510006

出　　处：《中药新药与临床药理》2015年第5期698-701,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(2011A030100014)

摘　　要：目的 采用顶空气相色谱法测定白芍、甘草提取物中因大孔吸附树脂带入的正己烷、苯、甲苯、乙基苯、二甲苯（间二甲苯,对二甲苯,邻二甲苯）、苯乙烯、二乙烯基苯类等有机溶剂残留量。方法 采用CP-WAX毛细管（30 m×0.25 mm,0.25μm）色谱柱;氢火焰离子化检测器;程序升温;进行顶空气相色谱测定。结果 9种有机溶剂完全分离,在所考察的浓度范围内相关系数均大于0.999以上;回收率为94.7%∽106.9%;检测限为0.03∽0.20μg·m L-1。结论 该方法简便易行,测定结果准确可靠,可作为白芍、甘草提取物中大孔吸附树脂残留物的测定方法。Objective To establish a method for the ethylbenzene, xylene（m-xylene, p-xylene, o-xylene）, determination of residues of n-hexane, benzene, toluene, phenylethylene, divinylbenzene brought by macroporous resin in the extraction of Radix Paeoniae Alba and Radix Glycyrrhizae through capillary gas chromatography with headspace sampling. Methods CP-Wax （30 m × 0.25 ram, 0.25 Ixm） capillary column and flame ionization detector were used for the temperature-programmed gas chromatography determination. Results Nine organic solvent residuals were completely separated. The correlation coefficients of linear range were over 0.999. The recovery rate was in the range of 94.7 %-106.9 %. The limit of detection ranged from 0.03-0.20 μg·mL-1. Conclusion The established method is accurate and quick with high sensitivity for the determination of organic residues of macroporous resin used in the extraction of Radix Paeoniae Alba and Radix Glycyrrhizae.

关 键 词：白芍 甘草 提取物 大孔吸附树脂 有机溶剂残留量 顶空气相色谱法 

分 类 号：R284.1[医药卫生—中药学]