油桐尺蛾核型多角体病毒广西株全基因组序列  

The Complete Genome of Buzura suppressaria Nucleopolyhedrovirus Guangxi Strain

作　　者：罗辑[1,2] 周国英[1] 朱积余[2] 

机构地区：[1]中南林业科技大学林学院,湖南长沙410004 [2]广西壮族自治区林业科学研究院,广西南宁530002

出　　处：《林业科学研究》2015年第5期612-617,共6页Forest Research

基　　金：国家自然科学基金项目(31400549);中国博士后科学基金资助项目(2014M552533XB);广西博士后专项资助项目;广西林科院基金项目(林科201401号)

摘　　要：油桐尺蛾核型多角体病毒广西株基因组序列由Sanger测序的方法得到。拼接后获得了121 268 bp序列,GC含量为36.76%。以长度不小于50 aa的序列认定为功能基因,共预测到131个开放阅读框,通过Blast比对,其中87个能注释到功能基因。对基因组序列进重复序列分析,共发现SSR位点174个,同源重复序列1个。在SSR位点中,包含6种长度的重复基序,且以富含AT的重复基序为主。同源重复序列两端为一59 bp片段或其一部分组成的重复序列,两端分别重复12次和7次,中间则为一段与重复片段无关的236 bp片段。与武汉株相比,广西株病毒在37个核心基因中有4个存在蛋白序列长度上的差异,而在其他非核心基因中有6个基因仅存在于一个病毒株中,另有13个基因存在蛋白序列长度上的差异。The genome of Buzura suppressaria nucleopolyhedrovirus isolate Guangxi was sequenced by Sanger＇ s method. The size of the genome is 121 268 bp with 36.76% G ＋ C content. It contains 131 hypothetical open read- ing frames （ORFs） that potentially encode at least 50 amino acids respectively. 87 ORFs were annotated by Blast. 174 SSR sites and one homologous repeated sequence （HRS） were found in the genome. The SSR sites contained 6 types of repeats, and most of them were AT-rich sequences. HRS contained 12 repeats at 5 ＇ end and 7 repeats at 3 ＇ end. The repeat was 59 bp or truncated. Between 5＇ end and 3 ＇ end rapeats, there was a 236 bp sequence with no obvious features. Compared with Wuhan strain, 4 genes among 37 core genes were different in protein length and 6 non-core genes were found only in one strain, and another 13 genes were different in protein length.

关 键 词：油桐尺蛾 核型多角体病毒 株系 基因组 重复序列 

分 类 号：S763.3[农业科学—森林保护学]