转基因水稻cry1Ab/Ac基因及蛋白在肉鸡肠道中的残留检测  

Detection of cry1Ab/Ac Gene and Protein Remained in Gut of Broiler Feeding Transgenic Rice

作　　者：徐灯[1,2] 耿丽丽[2] 张敏红[3] 卢凡[1] 张杰[2] 

机构地区：[1]湖北工业大学资源与环境学院,武汉430068 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193 [3]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《生物技术通报》2015年第9期84-90,共7页Biotechnology Bulletin

基　　金：转基因生物新品种培育重大专项(2012ZX08011001-005)

摘　　要：旨在研究转基因水稻对饲喂肉鸡的影响,以含有转cry1Ab/Ac基因水稻的饲料饲喂肉鸡,分析了肉鸡空肠、回肠、盲肠、直肠中cry1Ab/Ac基因和蛋白残留情况,并用体外实验验证了外源基因的降解情况。结果表明,饲喂转cry1Ab/Ac基因水稻21 d和42 d的肉鸡肠道4个部位中没有检测到cry1Ab/Ac基因残留,体外消化外源基因的实验发现外源基因在肉鸡肠道中约4 h后开始逐步降解。对上述4个部位内容物进行ELISA和Western blot分析,均未检测到Cry1Ab/Ac蛋白残留。综合分析表明,转基因水稻中的外源基因和蛋白在肉鸡肠道中易被消化和降解。To analyze the effects of genetically modified rice on feeding broilers, cry1Ab/Ac gene and protein remained in the jejunum, ileum, cecum and rectum of broiler feeding transgenic rice were detected, and the degradation patterns were testified in vitro. The results showed that there were no residues of cry1Ab/Ac gene in 4 intestinal parts of broilers fed transgenic rice for 21 and 42 d, and cry1Ab/Ac gene gradually degraded after 4 h in the gut in vitro test. No residues of Cry1Ab/Ac protein were detected in contents of the above 4 parts of broilers using ELISA and Western blot. In conclusion, cry1Ab/Ac gene and protein of the transgenic rice were easily digested and degraded in broiler intestines.

关 键 词：转基因水稻 肉鸡 基因及蛋白残留 体外消化 

分 类 号：S831.5[农业科学—畜牧学]