山羊Apaf-1和Apaf-2基因的cDNA克隆、序列分析及组织表达  

c DNA Cloning,Sequence Analysis and Tissue Expression of A paf-1 and Apaf-2 Gene of Goats

作　　者：罗斌[1] 卢建远[1] 马力[1] 胡亮[1] 刘霜[1] 杨珂伟 字向东[1] 

机构地区：[1]西南民族大学国家民委动物科学重点实验室,成都610041

出　　处：《生物技术通报》2015年第9期125-130,共6页Biotechnology Bulletin

基　　金：西南民族大学研究生创新型科研项目(CX2014SZ71)

摘　　要：采集5只多胎金堂黑山羊和5只单胎藏山羊在发情期的卵巢、垂体等组织样,进行Apaf-1和Apaf-2基因的c DNA克隆、序列分析,以及定量PCR技术对其mRNA进行组织表达量研究。结果表明,克隆出Apaf-1基因长度为3 750 bp,编码1 249个氨基酸,Apaf-2基因编码区全长为318 bp,编码105个氨基酸。这两个基因在两种山羊中序列相同,没有突变,且在5种组织中的表达均无差异。表明凋亡基因Apaf-1和Apaf-2在动物的进化中比较保守,与山羊多羔性状的相关性需要进一步研究。We collected the pituitary, ovary and other tissue samples of 5 Tibetan goats and 5 Jintang black goats at estrus stage, cloned cDNA of Apaf-1 and Apaf-2, analyzed their sequences, and investigated their mRNA expression of tissues by qPCR. The results showed that：the coding region sequence（CDS）of goat Apaf-1 gene was 3 750 bp long encoding 1 249 amino acids, and the CDS of Apaf-2 gene was 318 bp encoding 105 amino acids. The two genes showed the same sequences in both of goats, no mutation and no differential expression in 5 tissues. Our data suggested that gene Apaf-1 and Apaf-2 in animal’s evolution were conservative, and the correlation with the trait of goat’s prolificacy needs further studies.

关 键 词：山羊 APAF-1基因 Apaf-2基因 定量PCR 

分 类 号：S827[农业科学—畜牧学] Q78[农业科学—畜牧兽医]