檀香炭疽病拮抗细菌的分离筛选及鉴定  

Isolation,Screening and Identification of Bacteria Antagonistic to Colletotrichum fructicola

作　　者：田媛媛[1] 周国英[1] 左杰[1] 刘倩丽 杨权[1] 

机构地区：[1]中南林业科技大学经济林培育与保护省部共建教育部重点实验室,长沙410004

出　　处：《生物技术通报》2015年第9期158-162,共5页Biotechnology Bulletin

基　　金：国家林业公益性行业科研专项(201304402)

摘　　要：旨在从海南省国营澄迈林场的土壤中筛选出对檀香炭疽病具有较强生防效果的拮抗细菌。采集澄迈林场檀香不同种植区中优良单株的根际土壤,通过稀释平板法从中分离出71株细菌,以檀香炭疽病原菌为靶标菌,通过平板对峙法初筛和发酵液法复筛,获得一株具较强拮抗效果的细菌,编号为TXJ2-6;抗菌谱测定表明,该拮抗菌株对降香黄檀炭疽病、油茶软腐病、油茶叶枯病、油茶根腐病、油茶炭疽病5种病原真菌也具有明显拮抗效果;通过形态学观察、生理生化特性及16S r DNA序列分析,鉴定该拮抗菌株为甲基营养型芽孢杆菌(Bacillus methylotrophicus)。It is aimed to obtain a bacterial strain that has strongly antagonistic activity to Colletotrichum fructicola. Collecting rhizosphere soil from different superior individual of Sandalwood in different parts of Chengmai Forest Farm in Hainan Province and using dilution culture method, 71 strains of bacteria were isolated. Then using C. fructicola as the indicating bacterium, primary screening by flat confrontation and re-screening by fermentation liquid, one strain of bacterium with promising antagonistic activity was obtained, numbered as TXJ2-6. Antibacterial spectrum tests showed it had significantly antagonistic activities to 5 plant pathogens（Colletotrichum gloeosporioides, Agaricodochium camellia, Fusarium proliferatum, Pestalotiopsis microspora, and Colletotrichum gloeosporioides）. Based on the morphological, physiological and biochemical characteristics and 16S rDNA phylogenetic analysis, strain TXJ2-6 was identified as Bacillus methylotrophicus.

关 键 词：檀香 炭疽病 拮抗细菌 筛选 鉴定 

分 类 号：S763.7[农业科学—森林保护学]