补精益视片对大鼠慢性高眼压模型视网膜损害的影响  

Effects of Bujingyishi tablet on retinal damage in rat model of chronic elevated intraocular pressure

作　　者：李翔[1] 王桃[1] 柯欣怡 

机构地区：[1]成都中医药大学附属医院眼科,四川省成都市610072

出　　处：《眼科新进展》2015年第10期909-912,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:81373695/H2713);成都中医药大学科技发展基金(编号:030018024)~~

摘　　要：目的观察补精益视片对大鼠慢性高眼压(elevated intraocular pressure,EIOP)模型视网膜损害的干预作用,探讨其作用机理。方法将30只SD大鼠随机分为3组:对照组、给药组、模型组,每组10只。采用烙闭上巩膜静脉法对给药组、模型组SD大鼠建立慢性EIOP模型,观察补精益视片对慢性EIOP大鼠眼压和视网膜病理形态学变化的影响。结果给药组、模型组大鼠在造模后即刻直至造模后8周与造模前相比眼压均升高,差异均有统计学意义(均为P<0.01),说明EIOP模型造模成功。造模后8周眼压与造模后即刻相比,给药组有降低趋势,差异有统计学意义(P<0.01),模型组差异无统计学意义(P>0.05)。造模后8周,视网膜神经节细胞(retinal ganglion cells,RGCs)数量给药组(14.57±1.97)与模型组(10.76±2.19)均明显低于对照组(17.47±1.97),差异均有统计学意义(P<0.05,P<0.01);视网膜厚度模型组为(150.83±17.91)μm低于对照组的(219.72±32.24)μm,差异有统计学意义(P<0.01),给药组为(215.51±51.23)μm,与对照组相比差异无统计学意义(P>0.05);给药组RGCs数量及视网膜厚度均优于模型组,差异均有统计学意义(均为P<0.01)。RGCs超微结构显示给药组较模型组明显改善。结论补精益视片能保护SD大鼠慢性EIOP模型视功能,表现为降低眼压、提高RGCs数量、增加视网膜厚度、改善RGCs超微结构。Objective To observe the effects of bujingyishi tablet on the retinal damage in rat model of elevated intraocular pressure （ EIOP）, and preliminarily explore its mechanism. Methods Thirty SD rats were randomly and equally divided into 3 groups：control group,model group and treatment group, l0 rats in each group. By uni- laterally cauterizing three episcleral vessels, the rat model of chronic EIOP was estab- lished on model group and treatment group. The effects of bujingyishi tablet on intraoc- ular pressure （IOP） and pathological changes of retina were observed. Results Com- pared with pre-operation, IOP in treatment group and model group at models building until postoperative 8 weeks increased （ all P 〈 0.01 ）, indicated that the EIOP models were successfully established. There was obviously reduce in treatment group between postoperation 8 weeks and models buiding（P 〈 0.01 ） ,while model group was not statis- tically significant （P 〉 0.05 ）. At postoperative 8 weeks, the nμmber of retinal ganglion cells（RGCs） in treatment group（ 14.57 -＋ 1.97 ） and model group （ 10.76± 2. 19 ） were lower than that in control group（ 17.47 ± 1.97 ） （ P 〈 0.05 ,P 〈 0.01 ）. The retinal thick- ness had statistically significant difference between control group （219.72 ± 32.24 ）μm and model group （ 150.83 ± 17.91 ）μm （P 〈 0.01 ）, While there was not statistically signif- icant between control group and treatment group （215.51± 51.23 ）μm（P 〉 0.05 ）. The nμmber of RGCs and retinal thickness in treatment group were better than those in model group（ all P 〈 0. 01 ）. The improvement of RGCs ultrastructure in treatment group was better than that in model group. Conclusion Bujingyishi tablet can protect the visual function in the rat model of chronic EIOP, can decrease IOP, increase the nμmber of RGCs and retinal thickness,improve the ultrastructure of RGCs.

关 键 词：青光眼 补精益视片 大鼠 慢性高眼压模型 视神经 视网膜 

分 类 号：R988.1[医药卫生—药品]