大豆油分相关分子标记的实用性分析与验证  

Practical Analysis & Verification of Molecular Marker of Oil in Soybean

作　　者：任海红[1] 刘学义[1] 朱保葛[2] 林汉明[3] 

机构地区：[1]山西汾阳山西省农业科学院经济作物研究所,032200 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]香港中文大学生命科学学院,中国香港999077

出　　处：《作物杂志》2015年第5期37-40,共4页Crops

基　　金：国家大豆产业技术体系(CARS-04);山西省“百人计划”项目;山西省留学人员科研资助项目(2013-137)

摘　　要：2013年从各地收集到不同生态类型高油大豆品种37份、低油大豆品种11份,以此为材料对已经定位的19对SSR分子标记进行实用性验证研究,进而筛选实用分子标记和带有重要标记的育种材料,寻求分子标记辅助育种技术突破。参试样品DNA经PCR扩增和9%聚丙烯酰胺凝胶电泳,然后统计每个SSR位点带型,最后采用SPSS软件作方差分析。结果选出2个与高油相关的实用性标记,分别为Satt162和GNE165,Satt162是最理想的高油分子辅助育种可实用标记。可以被1个或2个标记检出的高油材料有35份(高油材料共37份),检出率高达94.6%。该试验结果表明Satt162和GNE165联合检测可以应用于分子标记辅助育种高油育种实践。Forty-eight soybean （thirty-seven high oil varieties and eleven low oil varieties） varieties were collected, including different ecological types. Ninteen pairs of position-known molecular markers were used for systematically practical confirmation and evaluation for breeding application. The purpose of present study was to screen useful molecular markers and breeding materials, and to seek further technology breakthrough in molecular marker-assisted breeding. The results were as follows：two practical molecular markers for breeding application （Satt162 and GNE165 ） were selected in the high/low oil groups,respectively. In short, thrity-seven varieties could be detected by one or two markers （the number of high oil material is thirty-seven） ,and the coincident rate was as high as 94. 6%. The results showed that the joint use of Satt162 and GNE165 could facilitiate the molecular marker-assisted breeding for high oil breeding in soybean.

关 键 词：大豆 油分 分子标记 实用性 

分 类 号：S565.1[农业科学—作物学]