慢性硝酸钐暴露对大鼠血流动力学及心脏组织结构与部分血液指标的影响  

Effects of Chronic Samarium Nitrate Exposure on Hemodynamics and Structure of Heart,Blood Index on Rats

作　　者：阮琴[1] 申秀英[2] 刘庆伟[3] 王芳[1] 徐晓虹[4] 俞世钢[1] 饶和平[5] 姜科声[4] 

机构地区：[1]浙江师范大学行知学院,浙江金华321004 [2]浙江科技学院生物与化学工程学院,浙江杭州310023 [3]金华市中心医院病理科,浙江金华321000 [4]浙江师范大学化学与生命科学学院,浙江金华321004 [5]衢州职业技术学院医学院,浙江衢州324000

出　　处：《中国稀土学报》2015年第5期624-632,共9页Journal of the Chinese Society of Rare Earths

基　　金：浙江省教育厅项目(Y201329454);浙江省自然科学基金项目(Y5110135)资助

摘　　要：探讨Sm(NO3)3慢性暴露对大鼠血流动力学、心脏组织结构及血常规指标的影响。SD大鼠48只,雌雄各半,随机分成4组,分别自由饮用0,3,4.5,6 mg·L-1Sm(NO3)3溶液180 d。开胸取心时采血进行血常规测定,心脏光镜切片观察心肌组织形态学的变化;部分心脏离体后应用Langendorff离体心脏灌流模型,观察Sm(NO3)3慢性暴露对大鼠心脏功能的影响。结果显示3 mg·L-1组LVSP,+dp/dtmax极显著地增高(p<0.01),CF显著下降(p<0.05);但4.5,6 mg·L-1组LVSP显著增高(p<0.05),LVEDP,+dp/dtmax,-dp/dtmax,HR,CF等指标无明显改变;3,4.5 mg·L-1Sm(NO3)3组WBC,MID,Lym数与对照相比有极显著和显著增高(p<0.01,p<0.05)。3 mg·L-1组心肌充血出血,肌束呈波浪状,肌细胞体积增大,胞浆内呈红染的颗粒状变性,核轻度增大。4.5 mg·L-1组心肌内皮细胞增生,小灶肌束断裂,部分细胞颗粒状变性。6 mg·L-1组心肌肌束排列紊乱,微灶细胞变性坏死,细胞核消失,间质少量淋巴细胞浸润。以上结果表明一定浓度Sm(NO3)3对离体大鼠心脏有正性变力作用;增加WBC,MID,Lym数,并可导致心肌组织的代偿性增生与损伤。To investigate the effects of chronic exposure to Sm（ NO3 ）3 on hemodynamics and the structure of heart and blood index of rats. SD rats were randomly assigned to 4 experimental groups （ n = 12） with free drinking water containing Sm（ NO3 ）3 at 0, 3, 4.5, and 6 mg L-1 for 180 d. And then Blood routine was tested, the structure of heart and the effect of chronic exposure to Sm（ NO3 ） 3 on heart function were examined by Langendorff isolated heart perfusion model. The results showed that 3 mg L-1 Sm （NO3 ）3 significantly increased the left ventricular systolic pressure （LVSP） and maximum rate of rise of pressure （ ＋ dp/dtmax ） （ P 〈 0.01 ）, but significantly decreased cor- onary flow （CF） （ P 〈 0.05 ）. Sm （ NO3 ） 3 at 4.5 and 6 mg L - 1 markedly increased LVSP （ P 〈 0.05 ）, while no obvious changes were found in left ventricular end-diastolic pressure （ LVEDP）, ＋ dp/dtmax, decline of pressure （ -dp/dtmax）, heart rate （HR）, and CF. Sm（N03）3 at 3 and 4.5 mg.L-1 significantly increased total white cell count （ WBC ）, neutrophil and lymphocyte count （ MID and Lym） （ P 〈 0.01, P 〈 0.05 ）. The 3 mg L- 1 group my- ocardial congestion or hemorrhage, wavy muscle, muscle cell volume increased, the cytoplasm were stained red granular degeneration and nucleus slightly increased. The 4.5 mg L-1 group myocardial endothelial cell prolifera- tion, focal muscle rupture and granular degeneration of some cells. The 6 mg.L-1 group cardiac muscle bundles de- rangement, micro tumor cell degeneration and necrosis, nucleus disappear. These results suggested concentration WBC, MID, of Sm （ NO3 ） 3 and Lym. and has positive inotropic effect on isolated rat hearts, and can increase the can lead to myocardial tissue damage.

关 键 词：硝酸钐 慢性暴露 血流动力学 心脏组织结构 血常规 稀土 

分 类 号：R994.6[医药卫生—毒理学] R977.5[医药卫生—药学]