外源GA4处理解除果梅花芽休眠的生理效应研究  

Physiological Effect of Exogenous GA4 on the Dormancy Release of Japanese Apricot Flower Buds

作　　者：温璐华 王真[1] 庄维兵[1] 王淼[1] 朱銑秋 李百健 高志红[1] 朱銑秋 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]南京傅家边科技园集团有限公司,南京211221

出　　处：《中国南方果树》2015年第5期16-22,共7页South China Fruits

基　　金：高校基本业务费项目(KYZ201208);江苏省青蓝工程;国家大学生科研训练项目(SRT)资助

摘　　要：为分析外源GA4解除果梅花芽休眠的生理效应,以高需冷量果梅品种"丰后"为试材,于果梅树体自然休眠期多次采集枝条,研究不同浓度GA4处理和质量分数为1%的外源H2O2处理对人工气候室内水培枝条萌芽率和H2O2及抗氧化相关酶类活性的影响。结果表明,GA4200μM处理的萌芽率明显高于质量分数为1%的H2O2处理和对照,1%H2O2对休眠解除效果不明显;GA4处理的最佳浓度为200μM,最佳处理时期为元旦前后;在GA4处理促进果梅在休眠解除期间,其花芽内的H2O2和相关抗氧化酶发生规律性变化,其中,H2O2含量在果梅花芽休眠解除时达到峰值,并且,促进H2O2产生的超氧化物歧化酶(SOD)活性也在此时达到峰值。过氧化氢酶(CAT)和过氧化物酶(POD)具有清除H2O2的作用,二者的活性分别在H2O2含量到达峰值之前和到达峰值之时受到抑制。总之,基于对H2O2可能在休眠解除期间作为信号物质的认识,根据这些酶的含量变化推测GA4打破果梅花芽休眠可能通过抗氧化酶系统调控H2O2含量的变化来对休眠解除起作用。To analysis physiological effects of exogenous GA4 on the dormancy re- lease of Japanese apricot （Prunus rnume Sieb. et Zucc）flower buds,Japanese apricot cultivar ＇Bungo＇ was used as experimental material. The branches were collected during endodormancy,treated with GA4 in different concentrations and 1% H2 02 in growth chamber. The changes of H202 content, germination rates and activities of antioxidant enzymes of the flower buds were investigated. The results showed that flower buds under GA4 treatment had a higher germination rate than 1% H2O2 treatment and control. The 1% H202 treatment had no obvious effect compared with control. The best concentration of GA4 for improving germination rate was 200 μM, and the best time was near the New Year＇s Day. There was a transient peak of H202 content during dormancy release, at the same time the activity of SOD also reached to the maximum of the whole experiment process. SOD was known as hav- ing an effect of inducing production of H2 02. CAT and POD could scavenge H2 02. Their activities were inhibited for a period of time during this experiment. The difference was that the activity of CAT was inhibited before dormancy release and the activity of POD was inhibited during dormancy release. According to these changes and also known that H2 02 acts as a signal in dormancy release, we sup- posed that GA4 can regulate H2 02 content via antioxidant enzyme system to release the dormancy.

关 键 词：GA4 POD CAT SOD 果梅 花芽 休眠解除 

分 类 号：S662.4[农业科学—果树学]