PfCP-2.9/PfCSP-2疟疾联合疫苗免疫效果血清学评价研究  

Serological evaluation on the immunization effect of PfCP-2.9/PfCSP-2 combination malaria vaccine

作　　者：杨英超[1] 王国柱[1] 李喆[1] 张金龙[1] 辛晓芳[1] 

机构地区：[1]中国食品药品检定研究院

出　　处：《国际医学寄生虫病杂志》2015年第5期261-264,共4页International JOurnal of Medical Parasitic Diseases

基　　金：国家高技术研究发展计划(863计划)(2012AA02A402)~~

摘　　要：目的：对PfCP-2.9/PfCSP-2疟疾联合疫苗所诱发的特异性IgG抗体进行分析评价。方法将5只新西兰白兔分为两组，其中免疫组3只，非免疫组2只。免疫组接种PfCP-2.9/PfCSP-2疟疾联合疫苗，非免疫组注射生理盐水。连续3次免疫后，采集血液样本并经间接ELISA检测其IgG抗体水平。根据非免疫组兔的吸光度数值（A值）计算临界值。临界值=2.1＆#215;非疫苗免疫组兔A值均值。若免疫组兔吸光度值高于临界值，则被判定为疫苗可诱发特异性 IgG 抗体。结果疫苗可诱发针对PfCP-2.9和PfCSP-2重组蛋白IgG抗体，经4个稀释浓度（1∶100、1∶200、1∶400、1∶800）检测获得2只免疫组兔A值分别为0.629/0.544、0.548/0.443、0.479/0.378、0.410/0.281，均高于相应稀释浓度的临界值（0.019、0.017、0.016、0.020）；疫苗可诱发针对 PfCSP 的特异性 IgG 抗体（每孔包被4μg/ml 多肽），经4个稀释浓度（1∶100、1∶200、1∶400、1∶800）检测2只免疫组家兔A 值分别为0.308/0.152、0.194/0.080、0.110/0.040、0.060/0.025，均高于相应稀释浓度的临界值（0.027、0.019、0.018、0.018）；疫苗可诱发针对PfCSP的特异性IgG抗体（每孔包被10μg/ml多肽），经4个稀释浓度（1∶100、1∶200、1∶400、1∶800）检测获得2只免疫组家兔 A 值分别为0.333/0.129、0.221/0.086、0.133/0.048、0.075/0.029，均高于相应稀释浓度的临界值（0.048、0.038、0.036、0.036）；结论 PfCP-2.9/PfCSP-2疟疾联合疫苗可诱发高水平IgG抗体，该疫苗是安全、有效且耐受性良好。Objective To analyze and evaluate the specific IgG antibodies level induced by PfCP-2.9/PfCSP-2 combination malaria vaccine. Methods Five New Zealand white rabbits were divided into two groups, three in vaccine immunized group, and two in non-vaccine immunized group. Vaccine immunized group was inoculated PfCP-2.9/PfCSP-2 combination malaria vaccine, non-vaccine immunized group was in-jected with saline. After 3 consecutive immunizations, blood samples were collected and tested by indirect ELISA to detect IgG antibody. Cut-off values were calculated in accordance with the absorbance value （A val-ue） obtained from non-vaccine immunized rabbits. Cut-off value=2.1× average A value of two non-vaccine im-munized rabbits. If absorbance values acquired from immunized rabbit were higher than the cut-off value, it was judged that the vaccine can induce specific IgG antibodies. Results Vaccines can induce specific IgG antibody against PfCP-2.9 and PfCSP-2 recombinant protein, after 4 level of serum dilution （1∶100, 1∶200, 1∶400, 1：800） detection, The A values from two vaccine immunized rabbits were 0.629/0.544, 0.548/0.443, 0.479/0.378, 0.410/0.281, higher than the cut-off value of the corresponding dilution （0.019, 0.017, 0.016, 0.020）. Vaccine can induce specific IgG antibodies against PfCSP （per well coated 4 μg/ml polypeptide）, af-ter 4 levels of serum dilution （1∶100, 1∶200, 1∶400, 1∶800） detection, The A values from two vaccine immu-nized rabbits were 0.308/0.152, 0.194/0.080, 0.110/0.040, 0.060/0.025, higher than the cut-off value of corre-sponding dilution （0.027, 0.019, 0.018, 0.018）;vaccine can induce specific IgG antibody against PfCSP（per well coated with 10 ug/ml polypeptide） , after 4 level of serum dilution（1∶100, 1∶200, 1∶400, 1∶800） detection, A values from two vaccine immunized rabbits were 0.333/0.129, 0.221/0.086, 0.133/0.048,0.075/0.029, higher than the cut-off value of corresponding dilution （0.048, 0.038, 0.036, 0.036）. Conclusion PfCP-2.9

关 键 词：疟疾疫苗 血清学 间接ELISA 

分 类 号：R511.2[医药卫生—内科学]