不同应激致郁证大鼠模型血管内皮功能的变化及调脂积的干预研究  

Vascular Endothelial Function Changes of Rats with Depression Due to Different Causes and Tiaozhiji Intervention

作　　者：唐黎群[1] 余肖琴 周宁[1] 

机构地区：[1]浙江省人民医院,浙江杭州310014 [2]杭州爱德医院,浙江杭州310014

出　　处：《中华中医药学刊》2015年第10期2415-2417,I0017,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省卫生厅项目(2012KYB017)

摘　　要：目的:观察不同应激方法制作中医郁证大鼠模型的可靠性,并了解调脂积对郁证大鼠模型血管内皮功能的影响。方法:Wistar老年大鼠共40只,随机分为正常空白组、慢性持续性温和刺激模型组、间断强刺激模型组、间断强刺激+调脂积干预组、慢性持续性温和刺激+调脂积干预组。适应性喂养后,给予除空白组外的其它组别予应激刺激(包括冰水游泳、热刺激、禁食、禁水、夹尾、昼夜颠倒、高速水平摇晃、频闪灯照射,但程度及频次不同),2个药物干预组在应激刺激的基础上喂服调脂积冲剂。之后观察各组大鼠体质量变化、糖水偏嗜等一般情况,同时检测血管活性物质(NO、ET-1)水平,电镜下观察主动脉超微结构。结果:给予不同应激刺激56 d后,2个模型组与空白组比较,糖水偏嗜度均有降低趋势,但无明显差异(P>0.05);2个药物干预组与对应模型组比较,糖水偏嗜度较模型组有明显升高,差异明显(P<0.05)。2个模型组与空白组比较,NO水平明显升高(P<0.05);2个药物干预组与对应模型组比较,NO水平有下降趋势,差异显著(P<0.05)。2个模型组与空白组比较,ET-1水平明显升高(P<0.05);2个药物干预组与对应模型组比较,ET-1水平呈下降趋势,但差异不明显(P>0.05)。同时间断强刺激模型组及间断间断强刺激+调脂积干预组大鼠血管内皮超微结构出现变化。结论:间断强刺激与慢性持续性温和刺激均可用于制作中医郁证模型,并可引起血管内皮功能的变化,可能以此途径影响心血管疾病的发病及预后;调脂积冲剂对大鼠的郁证及相关血管内皮功能及结构的有干预作用。Objective： To observe different stress method to make the reliability of the TCM depression syndrome rats model and to understand Tiaozhiji on vascular endothelial function of depression syndrome rats models. Methods ： 40 elderly Wistar rats were randomly divided into normal group, chronic persistent mild stimulation blank model group, strong stimulation model group, the jumps of strong stimulus ＋ Tiaozhiji intervention group, chronic persistent mild stimulation ＋ Tiaozhiji intervention group. Fitness after feeding, except the blank group, give the other groups stress （including water swimming, thermal stimulation, fasting, ban water, tail clip, day and night upside down, high - speed horizontal shaking, flashing light illuminate,but different degrees and frequencies）. Two drug intervention groups on the basis of the stress stimulus were given Tiaozhiji Granule. And then observe rats weight changes,partial addicted to sugar water, etc. Generally, at the same time detect vascular active substances （ NO and ET 1 ） level and observe the aortic uhrastrueture under electron microscope and the depressive effect of rat model of vascular endothelial function. Results ： Given different stress after 56 days ,2 model group compared with blank group, the sugar water partial heavy degrees are to reduce the trend, but no obvious difference （ P 〉 0. 05） ; Two drug intervention group compared with corresponding model group, the sugar wa- ter partial heavy degree has increased significandy in the model group, obvious difference （P 〈 0. 05）. Two model group compared with blank group, the NO level increased significantly （P 〈 0.05） ;Two drug intervention group compared with corresponding model group, there is a downturn in the NO level difference significant （P 〈 0.05 ）. Two model group com- pared with blank group, ET 1 level increased significantly （ P 〈 0.05 ） ; Two drug intervention group compared with corresponding model group,levels of ET- 1 is on the decline,but no obvious diffe

关 键 词：郁证 应激 血管内皮 

分 类 号：R258.8[医药卫生—中医内科学]