梓醇抑制AGEs诱导的EA.hy926细胞炎症反应及RAGE表达  

Inhibitory effect of catalpol on inflammation and expression of RAGE in EA. hy926 cells induced by advanced glycation end products

作　　者：刘江月[1] 

机构地区：[1]潍坊医学院病理生理教研室,山东潍坊261053

出　　处：《中国病理生理杂志》2015年第9期1693-1698,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.8130068);山东省自然科学基金资助项目(No.ZR2009CQ013);山东省中医药管理局资助项目(No.2013-237)

摘　　要：目的:研究梓醇对晚期糖基化终产物(AGEs)诱导的EA.hy926内皮细胞炎症反应的抑制作用并探讨其可能机制。方法:将常规培养的EA.hy926细胞随机分为对照组、梓醇对照组、AGEs组以及梓醇高剂量(0.5 mmol/L)、中剂量(0.25 mmol/L)和低剂量(0.05 mmol/L)保护组。激光共聚焦显微镜观察细胞内活性氧簇(ROS)的生成;RT-PCR和Western blot检测细胞中单核细胞趋化蛋白1(MCP-1)、肿瘤坏死因子α(TNF-α)、血管细胞黏附分子1(VCAM-1)及晚期糖基化终产物受体(RAGE)的mRNA及蛋白的表达。结果:梓醇保护组ROS生成均明显减少,MCP-1、TNF-α和VCAM-1的mRNA及蛋白表达均显著降低,RAGE蛋白表达明显受抑制,且呈剂量依赖性(P<0.05)。结论:梓醇能够有效抑制AGEs诱导的EA.hy926细胞内氧化应激,减轻炎症反应,其机制可能与其降低RAGE表达有关。AIM：To investigate the inhibitory effect of catalpol on inflammation in EA .hy926 cells induced by advanced glycation end products （ AGEs） and to explore its antioxidant mechanisms .METHODS：Human endothelial cell line EA.hy926 was cultured and randomly divided into control group , catalpol （0.5 mmol/L） group, AGEs group, high-dose （0.5 mmol/L） catalpol ＋AGEs group, middle-dose （0.25 mmol/L） catalpol ＋AGEs group and low-dose （0.05 mmol/L） catalpol＋AGEs group.Intracellular reative oxygen species （ ROS） production was detected by laser scanning confocal microscopy.The levels of monocyte chemotactic protein-1 （MCP-1）, tumor necrosis factor-α（TNF-α） and vas-cular cell adhesion molecule-1 （VCAM-1） in culture supernatant were detected by commercial ELISA kits .The expression of MCP-1, TNF-α, VCAM-1 and receptor for advanced glycation end products （RAGE） in the EA.hy926 cells were detec-ted by Western blot.RESULTS：In high-dose catalpol＋AGEs and middle-dose catalpol＋AGEs groups, the generation of ROS was decreased significantly .The levels of MCP-1, TNF-αand VCAM-1, and protein expression of MCP-1, TNF-αand VCAM-1 were significantly lower .The expression of RAGE protein in EA .hy926 cells were significantly inhibited （ P〈0.05）.CONCLUSION：Catalpol effectively inhibits the AGEs-induced oxidative stress and inflammation in EA .hy926 cells, which may be associated with a decrease in the expression of RAGE .

关 键 词：梓醇 氧化应激 炎症反应 晚期糖基化终产物受体 

分 类 号：R543.1[医药卫生—心血管疾病] R363[医药卫生—内科学]