原生质体融合选育耐高温柠檬酸生产菌  

High temperature resistant critic acid producing strain breeding by protoplast fusion

作　　者：徐凯[1] 张伟国[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《生物加工过程》2015年第5期26-30,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(31171640)

摘　　要：为获得耐高温柠檬酸生产菌,利用原生质体融合技术将具有优良性状的高产菌株W和耐高温柠檬酸生产菌Y-711进行融合,筛选耐高温的高产柠檬酸生产菌。通过变色圈筛选得到融合株R502,在40℃培养72 h就可产酸118.7 g/L,其产酸量比亲本菌株分别高10.5%和21.0%,培养时间分别减少14.3%和7.7%,该菌株遗传稳定。原生质体融合是一种有效获得耐高温柠檬酸生产菌的方法。To obtain high temperature resistant critic acid producing strain, the protoplast of high？yield strain W and high temperature resistant mutant strain Y？711 were fused. The obtained fusion strain R502 produced 118？7 g/L acid after cultivation at 40 ℃ for 72 h. The acid yield of stain R502 increased 10？5% compared with strain W and 21？0% compared with strain Y？711,respectively. Cultivation time reduced 14？3% compared to strain W and 7？7% compared to strain Y？711,respectively. The strain R502 was genetically stable. Protoplast fusion is an effective method to obtain a high temperature resistant citric acid producing strain.

关 键 词：原生质体融合 黑曲霉 耐高温柠檬酸生产菌株 

分 类 号：Q784[生物学—分子生物学]