姜黄素对肝星状细胞中髓样分化因子88蛋白表达的影响  

Effects of curcumin on the expression of myeloid differentiation factor 88 protein in hepatic stellate cells

作　　者：殷汉华 何雅军[2] 邓延梅[2] 潘立武[2] 刘序友[2] 韩馥缦 叶国荣[2] 吕霞[2] 舒建昌[2] 

机构地区：[1]广东省河源市中医院,1517000 [2]暨南大学医学院附属第四医院/广州市红十字会医院,2510220

出　　处：《现代消化及介入诊疗》2015年第4期339-342,共4页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：广东省科技计划项目(20120318023);广东省科技计划项目(2020314);中国肝炎防治基金会王宝恩肝纤维化研究基金项目(CFHPC20131014)

摘　　要：目的观察姜黄素干预前后肝星状细胞(HSC)中髓样分化因子88(My D88)蛋白的变化水平,以探讨姜黄素抗肝纤维化的相关机制。方法构建p CMV-Myc-My D88重组质粒,将人肾上皮细胞293T细胞及HSC-T6细胞分别设为空白对照组、My D88过表达组、姜黄素+My D88过表达组,其中My D88过表达组给予转染p CMV-Myc-My D88重组质粒72 h,姜黄素+My D88过表达组在转染48 h后加入姜黄素继续作用24 h。采用Western blot法检测My D88蛋白表达。结果 1转染p CMV-mycMy D88重组质粒48 h后,My D88在293T细胞及HSC中表达均明显增高(P<0.05),且经姜黄素处理后两类细胞中表达量明显降低(P<0.05)。2姜黄素处理后My D88表达量较My D88质粒转染组明显降低(P<0.05)。结论姜黄素可能通过抑制肝星状细胞My D88蛋白表达,阻断My D88依赖性信号通路的转导而发挥抗肝纤维化的作用。Objective To observe the expression difference of myeloid differentiation factor 88（MyD88） protein in hepatic stellate cell （HSC） treated with curcumin, and to investigate the related mechanism of the an-ti-fibrotic effect of curcumin. Methods 293T cell and HSC cell were individually divided into control group, vector of MyD88 plasmid group,and curcumin＋vectors of MyD88 plasmid group. The expression of MyD88 was detected by Western blot. Results ①After transfection of pCMV-Myc-MyD88, the expression of MyD88 was significantly increased （P〈0.05）, while the expression of MyD88 in curcumin＋vectors of MyD88 plas-mid group was significantly decreased than vectors of MyD88 plasmid group （P〈0.05）. ②After transfection of pCMV-Myc-MyD88 HSC, the expression of MyD88 was significantly increased （P〈0.05）. The expression of MyD88 in curcumin＋vectors of MyD88 plasmid group was significantly decreased compared with vectors of MyD88 plasmid group（P〈0.05）. Conclusions Curcumin might prevent liver fibrosis by inhabiting the ex-pression of MyD88 protein and blocking MyD88-dependent signaling pathway.

关 键 词：MYD88 姜黄素 肝星状细胞 

分 类 号：R811[医药卫生—放射医学]