贵阳地区2013年腹泻病例中致泻性大肠杆菌的病原监测分析  

Etiological surveillance of Diarrheagenic Escherichia coli from infectious diarrheal patients in Guiyang,China,2013

作　　者：韦小瑜[1] 游旅[1] 田克诚[1] 唐光鹏[1] 邹志霆[1] 王定明[1] 

机构地区：[1]贵州省疾病预防控制中心,贵阳550004

出　　处：《中国人兽共患病学报》2015年第9期881-885,共5页Chinese Journal of Zoonoses

基　　金：贵州省卫生厅科技基金(No.gzwkj2013-1-079);国家科技重大专项(No.2012ZX10004-212)联合资助~~

摘　　要：目的对贵阳地区2013年腹泻病例中致泻性大肠杆菌进行病原检测,为病例的确诊和贵阳地区致泻性大肠杆菌病疫情的预防和控制提供依据。方法收集2013年贵阳地区感染性腹泻病例粪便标本,接种麦康凯平板,初步生化符合大肠杆菌的菌落,采用多重PCR方法,进行致泻性大肠杆菌种属鉴定(uid基因)和毒力基因(bfpB、escV、stx1、stx2、elt、estIa、estIb、invE、astA、aggR、pic)检测并确定其致病型别。结果 257份腹泻病例粪便标本共检出致泻性大肠杆菌31株,检出率12.1%,其中EPEC11株,1株为典型EPEC,其余10株为非典型EPEC,EAEC15株,ETEC4株,EIEC1株,未检出EHEC。3岁及以下的婴幼儿和18岁及以上的成人是致泻性大肠杆菌感染的主要人群。结论贵阳地区致泻性大肠杆菌致病型别以EAEC、EPEC为主,婴幼儿和成人是致泻性大肠杆菌感染的主要人群,多重PCR检测方法是诊断致泻性大肠杆菌病的有效检测手段。Patients with infectious diarrhea of 2013 were etiologically detected in this study to provide scientific basis for the confirmation and prevention of the cases of diarrheagenic Escherichia coli in Guiyang, China. A total of 257 samples of stool specimens of diarrheal patients were collected. Each stool specimen was cultured by streaking on MacConkey agar for isolating diarrheagenic Escherichia coll. Isolates were conducted with biochemical identification and virulence genes detection by multi- plex PCR. The 31 diarrheagenic Escherichia coli strains were isolated from the total 257 specimens, accounting for 12. 1 ~. Among the 31 diarrheagenic Escherichia coli strains, there were 11 EPEC strains with 1 typical EPEC strain and 10 atypical EPEC strains, 15 EAEC strains, 4 ETEC strains and 1 EIEC strain. EHEC were not detected. Result shows that EAEC and EPEC were the dominant bacteria type among diarrheagenic Escherichia coZi, which spread mainly among infants aging younger than 3 and adults aging up to 18 in Guiyang. Multi-PCR methods play an important role in diagnosis of diarrheagenic Esche- richia coli disease.

关 键 词：感染性腹泻 致泻性大肠杆菌 多重PCR 毒力基因 

分 类 号：R378.2[医药卫生—病原生物学]