萼脊兰TUB基因片段的克隆及序列分析  

Cloning and Sequence Analysis of TUB Gene Fragment from Sedirea japonica

作　　者：蒋素华[1] 梁芳[1] 王洁琼[2] 李艳辉 王默霏[1] 崔波[1] 

机构地区：[1]郑州师范学院生物工程研究所,河南郑州450044 [2]河南农业大学生命科学学院,河南郑州450002 [3]封丘县农业局,河南新乡453300

出　　处：《华北农学报》2015年第4期31-34,共4页Acta Agriculturae Boreali-Sinica

基　　金：河南省科技攻关项目(092102110128);郑州市科技计划项目(112PPTGY250-3);校级项目(2012081)

摘　　要：为了给筛选萼脊兰内参基因和研究 TUB 基因的生理功能提供研究基础，对萼脊兰 TUB 基因片段进行克隆与序列分析。采用 CTAB 法提取萼脊兰盛花期花瓣总 RNA，并运用 RT-PCR 方法克隆萼脊兰 TUB 基因序列，长度为1055 bp，编码351个氨基酸残基，氨基酸序列中存在微管蛋白的保守区域 GGGTGSG。序列分析结果表明：与其他已登录物种的 TUB 基因相比，其核苷酸序列的同源性达75％～96％，与朵丽蝶兰（JN185665．1）同源性高达96％，氨基酸序列的同源性也在96％以上，且与单子叶植物的同源序列表现出较高的相似性。在 GenBank 注册，登录号为KP686397。TUB of Sedirea japonica was cloned and sequence analyzed,and the result would provide an impor-tant basis for selecting a reference gene and studying the function of TUB.The total RNA of Sedirea japonica scapes was extracted by CTAB method and the partial sequence of Sedirea japonica TUB was cloned by RT-PCR,which was 1 055 bp long and encoded 351 amino acids residues.Its amino acid sequence contained a conservative region GGGTGSG.The sequencing result revealed that the TUB gene fragment from Sedirea japonica homology comparison with other plants TUB gene sequences in the GenBank shows that they share 75%-96% nucleotide sequence ho-mology,96% nucleotide sequence homology with Doritaenopsis hybrid TUB,the amino acid sequence homology is a-bove 96% and amino acid sequence homology high similarity with the monocotyledon.The cloned genes were TUB gene fragment,which were registered them into GenBank（respective accession number：KP686397）.

关 键 词：萼脊兰 TUB 基因 克隆 序列分析 

分 类 号：Q786[生物学—分子生物学]