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作 者:邵彩虹[1,2] 王萍[1,2] 陈金[1,2] 李瑶[1,2] 刘光荣[1,2] 谢金水[1,2] 彭春瑞[1,2]
机构地区:[1]江西省农业科学院土壤肥料与资源环境研究所,江西南昌330200 [2]农业部长江中下游作物生理生态与耕作重点实验室,江西南昌330200
出 处:《华北农学报》2015年第4期51-58,共8页Acta Agriculturae Boreali-Sinica
基 金:国家科技支撑计划项目(2013BAD07B12;2011BAD16B04;2012BAD04B11);江西省自然科学基金项目(20132BAB204034);江西省农业科学院院创新基金项目(20121BBF60057)
摘 要:为揭示不易早衰水稻耐养分胁迫机理,应用蛋白质组学分析技术,对养分胁迫下不易早衰水稻隆平001生育后期叶片蛋白质组差异表达进行了研究。叶片蛋白质图谱经软件Imagemaster 2D Elite 5.0分析,有26个蛋白质发生差异表达,其中17个蛋白质功能经质谱分析(ESI-Q MS/MS)得到鉴定。差异表达蛋白质功能分析显示,养分胁迫下,隆平001叶片中光合及养分再利用相关蛋白质表达量增强,从而满足养分胁迫下植株生长发育的需要,是其具有较强抗养分胁迫的内在机理,而参与活性氧清除及逆境保护相关蛋白质表达量增强,可以保护光合机构免受胁迫伤害,从而对养分胁迫有更强的耐性、不易发生早衰。In order to search for the effects and the mechanism of nutrient stress on anti-senescence rice,the proteins expression profile of functional leaves at the booting stage of hybrid rice Longping 001 was studied by using the approach of plant proteomics.Proteins collected from Longping 001 leaves were separated with 2-DE and then analyzed with Imagemaster 2D Elite 5.0.Twenty-six differentially expressed proteins were obtained,among which 1 7 proteins were identified by ESI-Q MS /MS.The functions of these 1 7 proteins were analyzed and the results showed that:hybrid rice Longping 001 has strong adaptability to nutrient stress.The expression abundance of photosynthe-sis,nutrient-reused and stress resistance associated proteins increased in leaves,which should be beneficial to pro-duce more material for plant development and protect photosynthetic organism away from senescence.
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