高表达转 C4型 PEPC 基因水稻在低氮下诱导碳氮酶稳定光合作用  

Transgenic Rice with High Expression of C_4-PEPC Genes Induced Higher Carbon and Nitrogen Key Enzyme to Maintain Photosynthesis under Low Nitrogen Conditions

作　　者：唐玉婷[1,2] 李霞[2] 陆巍[1] 魏晓东[2] 

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心,江苏南京210014

出　　处：《华北农学报》2015年第4期95-100,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(No.31371554);江苏省自主创新基金项目(CX[(14)5004]);江苏省自然科学基金项目(BK20130708)

摘　　要：为揭示高表达转玉米C4-PEPC基因水稻在低氮条件下的光合表现,采用高表达转玉米C4-PEPC基因水稻(PC)与原种Kitaake(WT)为试验材料,通过盆栽试验,分别测定不同的氮素条件下(中氮300 kg/hm2,低氮65 kg/hm2),开花后不同天数的SPAD值,光合参数以及碳氮关键酶活性。并在开花后50 d收获植株,记录产量因子。结果表明:低氮处理下的PC相对于WT来说,在开花后14,28 d,其净光合速率分别提高了13.10%(P<0.05)和29.29%(P<0.05)。同时,Rubisco羧化酶在开花后14,28 d时活性分别提高了67.86%和52.63%(P<0.05);硝酸还原酶在14,28 d时的活性分别提高79.49%(P<0.05)和17.96%;谷氨酰胺合成酶仅在开花后28 d时活性提高28.48%(P<0.05)。但是通过对产量进行分析,在低氮条件下,并未发现PC的产量与WT有明显差异。可见,PC通过诱导碳氮的关键酶活性的提高维持低氮条件下高净光合速率。The objective of this study was to reveal transgenic rice with high expression of maize C 4-PEPC pho-tosynthetic performance under low nitrogen conditions,two rice genotypes including C 4-PEPC transgenic rice （PC） and untransgenic Kitaake rice（WT）in the pot experiments were chosen to measure their photosynthesis characteris-tics,SPAD values,PEPC,Rubisco,NR and GS activities of flag leaves at different flowering stage under different ni-trogen applications（normal nitrogen 300 kg /ha,low nitrogen 65 kg /ha）and their yield component were investigated after harvest.The results showed that the net photosynthetic rate of PC compared with WT were increased by 1 3.1 0% （P〈0.05 ）and 29.29% （P〈0.05 ）,respectively in the 1 4,28 d after flowering under low nitrogen treatment.At the same time,under low concentrations of nitrogen,relative to untransformed wild type rice,activity of Rubisco carboxylase of PC were increased by 67.86% and 52.63% （P〈0.05）in the 1 4,28 d after flowering re-spectively,while activity of nitrate reductase of PC were increased 79.49% （P〈0.05）and 1 7.96%.Meanwhile, activity of glutamine synthetase of PC was increased 28.48% （P〈0.05）only in the 28 days after flowering com-paring with WT.But we did not find significant differences in the yield of PC and WT under low nitrogen condi-tions.Therefore,PC maintain high net photosynthetic rate by inducing the activity of carbon and nitrogen key en-zyme improvement under low nitrogen conditions.

关 键 词：高表达转 C4 型 PEPC 基因水稻 低氮 光合特性 

分 类 号：S143.1[农业科学—肥料学] S511.01[农业科学—农业基础科学]