氢气对重度子痫前期胎盘滋养细胞增殖及凋亡的影响  

Effects of molecular hydrogen on the proliferation and apoptosis to severe preeclampsia trophoblast cells

作　　者：管中[1] 杨翔[1] 郭丽丽[2] 余慧玲[1] 李怀芳[1] 

机构地区：[1]同济大学附属同济医院妇产科,上海200065 [2]同济大学附属上海第一妇婴保健院妇产科,上海200040

出　　处：《现代妇产科进展》2015年第8期598-601,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金青年科学基金项目(No:81300500)

摘　　要：目的:探讨氢气对重度子痫前期(PE)胎盘滋养细胞的增殖和凋亡的影响。方法:MTT法检测不同浓度含氢培养基作用后滋养细胞的增殖活性。采用流式细胞术检测氢气作用24h后细胞凋亡率及细胞周期分布。结果:氢气能显著增强重度PE滋养细胞的增殖活性,抑制细胞凋亡(P<0.05),并使细胞周期中G1期比例减少,S期比例升高(P<0.05)。结论:氢气能显著提高重度PE胎盘滋养细胞的增殖活性,并能抑制细胞凋亡,促进细胞分裂。提示氢气在干预PE方面,可能是一种较为有效的抗氧化剂,具有潜在的预防和治疗价值。Objective：tion and apotosis to severe preeclampsia trophoblast cells. Methods：MTT was used to detect cell proliferation after trophoblast cells cultured by hrdrogen-rich medium. Flow cytometry was used to evaluate cell apotosis and cell cycle after treated by hydrogen-rich medium for 24h. Re-sults：Cell viability was significantly increased by 50 ～ 200μmol/ L molecular hydrogen （ P 〈0. 05）. Meantime molecular hydrogen can inhibit cell apotosis （P〈0. 05）,decrease the portion of G1 phrase cells （P〈0. 05） and raise the portion of S phrase cells （P〈0. 05）. Conclusion：Molecular hydrogen significantly increases the proliferation of severe preeclampsia trophoblast cells in vitro,inhibits cell apotosis and blocks the cell cycle at S phrase. It suggests that H2 acts as an effective anti-oxidant,may possess the potential for preventive and therapeutic applications in PE.

关 键 词：氢气 妊娠期高血压疾病 滋养细胞 细胞周期 凋亡 增殖 

分 类 号：R714.24[医药卫生—妇产科学]