荧光定量PCR检测女性生殖道解脲脲原体原体的应用评价  

Application of fluorescence quantitative PCR for detection of Ureaplasma urealyticum in female genital tract

作　　者：黄会[1] 赵香依 吴多荣[1] 姚敏[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院检验科,海南海口570208 [2]乐东县人民医院检验科,海南乐东572500

出　　处：《海南医学》2015年第18期2720-2722,共3页Hainan Medical Journal

摘　　要：目的：了解荧光定量PCR检测解脲脲原体（Uu）敏感性、特异性及其临床应用价值。方法收集海口人民市医院2013年3~5月妇产科门诊泌尿生殖道感染患者生殖道标本共154例，同时进行荧光定量PCR和液-固体培养法检测Uu，以液-固体培养法作为金标准，对荧光定量PCR诊断试验进行方法学评价。结果154例标本中，液-固体培养法即金标准检测阳性64例，荧光定量PCR检测阳性69例，两者同时阳性63例，同时阴性84例，荧光定量PCR检测Uu敏感性是98.44%（63/64），特异性是93.33%（84/90），假阳性率是6.67%（6/84），假阴性率是1.56%（1/64），阳性预测值是91.30%（63/69），阴性预测值是98.82%（84/85），总符合率是95.45%（147/154）。χ2检验显示，两种方法检测结果差异无统计学意义（P〉0.05），计算Kappa〉0.75，表明两种方法检测结果的一致性程度较好。结论荧光定量PCR方法具有灵敏度高、特异好、检测快速的优点，且与培养法一致性也较高，具有一定的临床应用价值。Objective To evaluate the sensitivity, specificity, and application of fluorescence quantitative PCR （FQ-PCR） for detection of Ureaplasma urealyticum （Uu） in female genital tract. Methods A total of 154 speci-mens of patients with genital tract infections were detected by FQ-PCR and liquid-solid culture at the same time. All specimens were collected in Haikou People＆#39;s Hospital from March 2013 to May 2013. Results In the 154 specimens, 64 and 69 samples were revealed as positive according to the golden standard method and FQ-PCR method respective-ly, with 63 samples positive and 84 negative for both the golden standard method and FQ-PCR method. For FQ-PCR, the sensitivity rate was 98.44%（63/64）, and the specificity rate was 93.33% （84/90）, with the false positive rate of 6.67%（6/84）, the false negative rate of 1.56%（1/64）, positive predictive value of 91.30%（63/69）, negative predictive value of 98.82%（84/85）, and the total coincidence rate of 95.45%（147/154）.χ2 test results showed that there was nostatistical significant difference between the results of the two methods （P〉0.05）, and the consistency was good （Kap-pa〉0.75）. Conclusion FQ-PCR has the advantages of high sensitivity, good specificity, rapid detection, and high con-sistency with culture, and thus has certain clinical application value.

关 键 词：荧光定量PCR 解脲脲原体 应用 

分 类 号：R711.3[医药卫生—妇产科学]