615例门诊就诊者HPV基因检测结果分析  

Human papillomavirus gene analysis of 615 out-patients

作　　者：林丽华[1] 钟娜[1] 乔凤[1] 张丽芬[1] 

机构地区：[1]海南省皮肤性病防治中心,海南海口570206

出　　处：《海南医学》2015年第18期2723-2724,共2页Hainan Medical Journal

摘　　要：目的了解海口地区人乳头瘤病毒(HPV)感染的情况,为HPV感染患者的治疗及监测提供科学依据。方法采用实时荧光定量PCR方法(FQ-PCR)对海口地区615例性病门诊就诊者泌尿系分泌物标本的HPV6,11型和HR-HPV(8个型)进行检测。结果 615例样本中检出HPV阳性者302例,感染率为49.11%(302/615),其中HPV6,11-DNA阳性130例,阳性率为21.14%(130/615),HR-HPV-DNA阳性126例,阳性率为20.49%(126/615),HPV6,11型合并HR-HPV阳性46例,占7.48%(46/615)。HPV6,11低危型与HR-HPV混合高危型比较差异无统计学意义(P>0.05)。302例HPV感染者中尖锐湿疣(CA)患者187例,占61.92%(187/302),检出HPV6,11-DNA阳性126例,占67.38%(126/187),HR-HPV-DNA阳性19例,占10.16%(19/187),HPV6,11型和HR-HPV混合感染42例,占22.46%(42/187)。CA患者感染以HPV6,11低危型为主,HPV6,11/HR-HPV次之,少部分为高危混合型HR-HPV感染,各组比较差异有统计学意义(P<0.01)。结论尖锐湿疣主要的基因型感染是低危型HPV6,11,高危型HPV感染多以混合感染形式存在。荧光定量PCR方法检测尖锐湿疣患者HPV基因型具有快速、准确的优点,是临床HPV感染分型检测的有效方法。Objective To explore human papillomavirus （HPV） infection in people in Haikou, China, and provide a basis for the treatment and monitoring of HPV-infected patients. Methods The urinary secretion speci-mens of 615 patients from Out-patient Department of Venereal Diseases were analyzed with real-time fluorescent quantitation PCR （FQ-PCR） for HPV6,11 and HR-HPV （8 types）. Results Of the 615 specimens, 302 （49.11%, 302/615） were positive of HPV, including 130 （21.14%, 130/302） positive of HPV6,11 DNA, 126 （20.49%, 126/302） posi-tive of HR-HPV DNA, and 46 （7.48%, 46/302） positive of both HPV6,11 and HR-HPV DNA. There was no statistical-ly significant difference between the low-risk group （HPV6,11） and the high-risk group （HPV6,11 combined with HR-HPV）, P〉0.05. Of the 302 HPV-infected patients, 187 （61.92%, 187/302） had condyloma acuminata （CA）, out of which 126 （67.38%, 126/187） were positive for HPV6,11 DNA, 19 （10.16%, 19/187） positive for HR-HPV DNA and 42 （22.46%, 42/187） positive for both HPV6,11 and HR-HPV DNA. Infection with low-risk HPV6,11 was mostly seen in these CA patients, followed by mixed infection with both HPV6,11 and HR-HPV and then by high-risk infec-tion with HR-HPV, where the differences among groups were significant （P〈0.01）. Conclusion The main genotype of CA is low-risk HPV6,11, while the high-risk genotype of HPV often exists in a mixed status. Being rapid and pre-cise in the determination of HPV genotype in CA patients, fluorescent quantitation PCR is an effective clinical method for classifying HPV infection.

关 键 词：人乳头瘤病毒 FQ-PCR 感染率 尖锐湿疣 

分 类 号：R373[医药卫生—病原生物学]