藏猪与雅南猪IGF-I基因的克隆与序列分析  

Cloning and sequence analysis of IGF-I gene in Tibetan pig and Yanan pig

作　　者：李伟[1] 张飞燕[1] 宋婷婷[1] 谷笑笑 刘红露[1] 潘大敏 韩书饼 潘康成[1,2] 

机构地区：[1]四川农业大学动物医学院动物微生态研究中心,四川成都611130 [2]动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《浙江农业学报》2015年第8期1324-1330,共7页Acta Agriculturae Zhejiangensis

基　　金："教育部长江学者和创新团队发展计划"创新团队资助项目(IRT0848);大学生创新训练计划项目(201410626028)

摘　　要：为研究IGF-I基因对藏猪、雅南猪生长发育的影响,以Gen Bank中猪IGF-I基因序列(登录号:DQ121132.1)设计引物,提取藏猪、雅南猪肝脏组织总RNA,应用RT-PCR技术克隆出IGF-I基因,分别将藏猪、雅南猪IGF-I基因克隆到PMD19-T载体上,构建重组质粒p MD19-T-IGF-I,经PCR鉴定、测序分析得该基因大小为612 bp,包含一个完整的ORF,该ORF共有462个碱基,编码153个氨基酸;所测得的藏猪和雅南猪基因序列与Muller等报道的猪IGF-I基因编码序列高度同源,序列比对发现,藏猪的同源性为100%,雅南猪的为99.61%;藏猪IGF-I与雅南猪IGF-I基因序列的同源性为99.61%;雅南猪在258 bp处发生A→G突变,但其编码的氨基酸未发生改变。To study the impact of IGF-I gene on the growth of Tibetan pig and Yanan pig, a pair of primers was de- signed by sus scrofa IGF-I gene sequence from GenBank （Accession number：DQ121132. 1 ）, the total RNAs were ex- tracted by using Trizol from the livers of Tibetan pig and Yanan pig and used as template to amplify IGF-I gene by RT-PCR,the amplified fragments were cloned into pMD19-T vector and the recombinant plasmids pMD19T-IGF-I were constructed and sequenced. The sequencing results indicated that the IGF-I gene consisted of 612 nucleotides, containing a complete ORF of 462 bp ecoding 153 amino acids. The two IGF-I gene sequences shared high homology with the porcine IGF-I gene reported by Muller, et al. , which had an identity of 100% in Tibetan pig and 99. 61% in Yanan pig. It shared 99. 61% homology between the Tibetan pig and Yanan pig IGF-I gene. An A→G mutation oc- curred in the 258 bp of Yanan pig, but the corresponding amino acid was not changed.

关 键 词：藏猪 雅南猪 IGF-I基因 序列分析 

分 类 号：S828.2[农业科学—畜牧学]