碳源对加纳链霉菌合成默诺霉素的影响  

Effect of carbon sources on moenomycin biosynthesis in Streptomyces ghanaensis

作　　者：车阳[1] 赵春田[1] 裘娟萍[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《浙江农业学报》2015年第8期1355-1361,共7页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金(Y2111182);浙江省生物工程重中之重学科开放基金(20130103)

摘　　要：通过摇瓶发酵测定加纳链霉菌发酵曲线以及补料前后默诺霉素合成基因转录水平分析,寻找适宜默诺霉素合成的补料碳源。结果发现,发酵至84 h,发酵液中残糖含量最低,在该时刻补加一定浓度碳源,测定效价可知,高浓度葡萄糖和蔗糖对默诺霉素的合成具有抑制作用,补加0.5%蔗糖能有效促进默诺霉素的合成,转录水平分析发现蔗糖的补加促进了默诺霉素合成相关基因的转录,默诺霉素效价提高14.3%。蔗糖的补加有助于促进默诺霉素生物合成中相关底物的合成,从而提高了默诺霉素发酵水平。The effective carbon sources were screened by the growth curve of Streptomyces ghanaensis and the tran- scription analyses of moenomycin biosynthetic genes. The results indicated that residual sugar concentration in the fermentation medium reached the lowest level at 84 h. At the moment, the addition of glucose and sucrose with high concentrations could obviously inhibit the moenomycin biosynthesis. The RT-PCR results showed that the transcrip- tion level of the moenomycin biosynthetic genes were promoted by the addition of sucrose. The final titer of moenomy- cin was improved by 14. 3% with the addition of 0. 5% sucrose. The addition of sucrose could promote the synthesis of related substrates involved in moenomycin biosynthesis. As a result, the moenomycin yield was improved.

关 键 词：默诺霉素 加纳链霉菌 碳源 蔗糖 转录分析 

分 类 号：S859.796[农业科学—临床兽医学] Q939.9[农业科学—兽医学]