草莓花青苷分子调控机理的初步研究  

Preliminary study on molecular regulation mechanism of anthocyanin biosynthesis in strawberry

作　　者：苗立祥[1] 荣宁宁[1,2] 张豫超[1] 杨肖芳[1] 张琴 蒋桂华[1] 

机构地区：[1]浙江省农业科学院园艺研究所,浙江杭州310021 [2]浙江师范大学化学与生命科学学院,浙江金华321004 [3]舟山市定海区农业技术推广中心站,浙江舟山316000

出　　处：《浙江农业学报》2015年第8期1373-1380,共8页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31201613);公益性行业(农业)科研专项(201003064);浙江省农业新品种选育专项(2012C12904-8);浙江省农科院创新提升工程(2012R05Y01E03)

摘　　要：为了初步阐明草莓花青苷合成调控的分子机理,文章从章姬、红颊、丰香、红花、越丽、越珠和10-26-77等草莓品种(系)中扩增了与调控草莓花青苷合成相关的MYB10和MYB1基因的c DNA和DNA全长以及MYB10基因的启动子序列。利用荧光定量PCR分析了章姬和10-26-77不同果实发育阶段的基因表达模式,利用高效液相色谱法(HPLC)分析了这2个草莓品种(系)在不同发育时期的花青苷含量。结果表明,不同草莓品种(系)的MYB10和MYB1基因在c DNA序列上没有差别,但DNA序列上存在SSR位点。MYB10在调节草莓果实花青苷合成代谢中起着决定性的作用,花青苷含量的变化与MYB10基因的表达变化趋势一致。不同草莓品种在MYB10转录因子基因启动子序列上的差异是导致基因转录调控不同的根本原因,从而调控草莓花青苷合成基因的转录表达水平,使得草莓花青苷含量有差异,呈现出颜色上的深浅。In order to elucidate the molecular mechanism of anthocyanin biosynthesis in strawberry, the DNA and eDNA sequences of MYBIO and MYB1 gene were amplified from seven strawberry varieties （lines） , Akihime, Beni- hoppe, Toyonaka, Honghua, Yueli, Yuezhu and 10-26-77. The promoter sequences of MYBIO and MYB1 gene were also amplified from genomie DNA. The gene expression patterns and anthoeyanin contents in different developmental stages of Akihime and 10-26-77 were screened using SYBR Green-I real-time fluorescent quantitative PCR and HPLC, respectively. The results showed that there was no difference in MYB10 and MYB1 genes at eDNA level among strawberry varieties, but there were different SSR sites in DNA sequences. MYBIO played a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening of Fragaria × ananassa fruits. And the anthoey- anin contents were consistent with changes in the MYB10 gene expression in different fruit stages. The difference inpromoter of MYB10 was the main cause of difference in gene expression, which led to the difference in the anthocya- nin content and fruit color of strawberry cuhivars.

关 键 词：花青苷 转录因子 草莓 

分 类 号：S668.4[农业科学—果树学]