胚胎培养液中能量底物对猪体细胞克隆胚胎体外培养的影响  

Effect of Energy Substrates in the Culture Medium on in Vitro Development of Porcine Somatic Cloned Embryos

作　　者：周荣[1] 罗绿花[1] 石俊松[1] 李紫聪[2] 王青来[1] 蔡更元[1] 吴珍芳[1,2] 

机构地区：[1]广东温氏食品集团股份有限公司国家生猪种业工程技术研究中心,广东云浮527439 [2]华南农业大学动物科学学院,广东广州510642

出　　处：《广东畜牧兽医科技》2015年第5期28-31,共4页Guangdong Journal of Animal and Veterinary Science

基　　金：创建肉鸡;肉猪种业国家工程技术研究中心能力建设(2012B07081006);广东省家畜分子与细胞工程育种技术研究团队(2011A020102003)

摘　　要：如何根据猪胚胎发育的不同阶段合理使用不同成分的培养液,建立高效的体细胞克隆胚胎体外培养体系。本研究把所获得的猪体细胞克隆胚胎用不同的培养液培养,比较体外囊胚发育力和囊胚细胞数。结果表明:PZM-3比NCSU-23更适用于克隆胚胎的培养;培养前4d使用PZM-3,后2d更换含有葡萄糖的NCSU-23,可以提高胚胎的囊胚细胞数。说明胚胎在不同发育阶段对能量底物的需求不同,合理利用可以有效提高胚胎发育力。Uusing the culture medium reasonably according to the different stages of the pig embryonic development, An efficient system of somatic cell cloned embryos in vitro culture was established. In this study,the porcine SCNT embryos were eultured with different culture medium, then compare the blastocyst formation rate and cell number per blastocyst Results showed that compared to NCSU-23,PZM-3 was better for the porcine SCNT embryos.The first 4d using PZM-3,then changed NCSU-23 containing glucose for another 2d,could improve the cell number of the blastocysts. The results showed that the demands for energy substrates were different depending on the developmental stage in pig embryos, rational using can improve the development of the embryos.

关 键 词：猪 体细胞核移植 培养液 能量底物 

分 类 号：S814.8[农业科学—畜牧学]