上皮内淋巴细胞在双峰驼小肠内的分布特征  

Distribution characteristics of intraepithelial lymphocytes in small intestine of Bactrian camel

作　　者：李淑娴[1] 郭明刚[1] 王雯慧[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2015年第4期18-22,共5页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(31260595;30671549);教育部博士点基金(200807330004)

摘　　要：试验采用HE常规染色方法,光镜下观察小肠各段肠黏膜上皮内淋巴细胞的分布位置、密度和形态,并对其进行计数,来探索双峰驼肠黏膜免疫机制.结果表明:上皮内淋巴细胞在小肠各段数量分布有显著差异(P<0.05),从十二指肠到回肠数量逐渐增多,每5 000μm2大小的肠黏膜上皮细胞中,肠上皮内淋巴细胞的平均数量分别为5.40、17.36和33.64个.上皮内淋巴细胞多数位于上皮吸收细胞的基底部,少数分布在上皮细胞核区和核上方,且后者多出现在空肠后段到回肠后段.上皮内淋巴细胞形态单一,多为圆形和椭圆形,与周围上皮吸收细胞的界限非常清晰,有一明显的"空晕".肠上皮内淋巴细胞从十二指肠到回肠数量逐渐增多,说明了上皮内淋巴细胞的归巢可能与小肠各段的抗原因素差异以及上皮吸收细胞基底侧的E-钙粘连蛋白表达密切相关.这一结果与在其他动物上的研究结果是不相同的.Using the HE regular dyeing method, the distribution location, density and morphology of the intraepithelial lymphocytes in small intestine were observed,and its number was counted under micro- scope. The results showed that the distribution characteristics of the intraepithelial lymphocytes among the different places of the small intestine were significantly different （P^0. 05）. The quantity of the intraepi- thelial lymphocytes increased gradually from duodenum to ileum. Within each 5 000 t^m2 intestinal mucosal epithelial ceils,the average number of intestinal intraepithelial lymphocytes was 5.40,17.36 and 33.64 re- spectively. Most of the intraepithelial lymphocytes were distributed in the base of absorption cell, and a small number of the intraepithelial lymphocytes were distributed near the free surface of epithelial cells. The latter was mainly distributed from the distal jejunum to distal ileum. The cell morphology was round and oval, it＇s boundary with the surrounding epithelial absorption cell was clear and had a ＇halo＇. The study showed that the quantity of intestinal intraepithelial lymphocytes from duodenum to ileum increased gradu ally, and the phenomenon further illustrated the homing of intraepithelial lymphocyte was closely related to the E-calcium adhesion proteins expressing on the epithelial basal side of absorptive ceils and the different antigens among the different places of the small intestine.

关 键 词：双峰驼 小肠 上皮内淋巴细胞 分布 

分 类 号：S824[农业科学—畜牧学]