甘肃省苹果树腐烂病菌ITS序列分析及潜育期研究  

Study on the incubation period and ITS sequence of pathogen of apple tree Valsa canker in Gansu Province

作　　者：张旭业 王卫雄[1] 张建华[1] 郑宇宇 候宝宏 陈臻 徐秉良[1] 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃省植检站,甘肃兰州730020

出　　处：《甘肃农业大学学报》2015年第4期80-85,89,共7页Journal of Gansu Agricultural University

基　　金：公益性行业(农业)科研专项(200903004);农业部公益性行业专项(201203034);国家苹果产业技术体系(CARS-28);国家星火计划项目(2013GA860001)

摘　　要：为了明确甘肃省苹果树腐烂病菌的种类及其优势种的潜育期,通过ITS序列比对和离体枝条接种法对采自甘肃省不同地区的苹果树腐烂病菌进行了ITS序列测定、分析及潜育期研究.结果表明:甘肃省苹果树腐烂病菌有2个种,分别为Valsa mali和Valsa malicola,其中Valsa mali有2个变种,分别为Valsa mali var.mali和Valsa mali var.pyri.甘肃省苹果树腐烂病菌优势种Valsa mali的潜育期随保湿时间增加而缩短,保湿时间为72h时潜育期为74.3h;温度30℃时潜育期最短,为64.3h;潜育期随枝龄的增加而增加,嫩梢的潜育期为46.3h;光暗交替下潜育期最短,为45.3h.In order to clarify incubation period and pathogen of apple tree Valsa canker in Gansu Prov- ince, the ITS sequence and incubation period of pathogen were studied by analysis ITS sequence and excised twigs inoculating. The results showed that there were two species of Valsa could cause this disease in Gan- su,Valsa rnali and Valsa rnalicola. The V. rnali could be also divided into two varieties,V, mall vat. mall and V. rnali var. pyri. Incubation period of apple Valsa canker shortened with the increasing of moisture time. When moisture time was 72 h, the incubation period was 74. 3 h. The incubation period was the shor- test at 30 ℃. The incubation period also prolonged when branch age increased, the incubation period of young shoots was 46.3 h. The incubation period under the alternation of light and darkness every 12 h was the shortest,that was 45.3 h

关 键 词：苹果树腐烂病菌 ITS 潜育期 甘肃 

分 类 号：S763.15[农业科学—森林保护学]