油茶组培苗高效增殖体系的建立  

Establishment of High Efficient Propagation System of Tissue Culture Seedling of Camellia oleifera

作　　者：王瑞[1,2,3] 陈永忠[2,3] 王湘南[2,3] 彭邵锋[2,3] 陈隆升[2,3] 马力[2,3] 罗健[2,3] 杨小胡[2,3] 

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]湖南省林业科学院,湖南长沙410004 [3]国家油茶工程技术研究中心,湖南长沙410004

出　　处：《中南林业科技大学学报》2015年第10期40-43,共4页Journal of Central South University of Forestry & Technology

基　　金：湖南省科技重大专项"油茶良种繁育与生态高效培育关键技术研究与示范"(2013FJ1006)

摘　　要：以油茶优良无性系"湘林1号"的茎段为外植体培养的无菌苗为材料,开展了无菌苗增殖培养研究,研究结果表明:基本培养基及外源激素均对油茶无菌苗增殖培养产生重要作用,以WPM为基本培养基,激素组合为6-BA5.0 mg/L+NAA0.01 mg/L+生物素1.0 mg/L时,增殖系数达最高值3.90;以MS为基本培养基,激素组合为6-BA1.0 mg/L+NAA0.01 mg/L+生物素0.5 mg/L时,新梢生长量达2.01 cm。Sterile seedlings of Camellia oleifera superior clones ＂XL1＂ , which induced from stem segments,were taking as materials, and proliferation culture were studied. The results showed that： basic medium and exogenous hormones played important roles in proliferation culture, the shoots were subcultured and multiplied on WPM medium containing 5.0 mg/L 6-BA, 0.01 mg/L NAA and 1.0 mg/L biotin, and proliferation coefficient reached 3.90; the young sprout of seedlings grew rapidly on MS medium containing 1.0 mg/L 6-BA, 0.01 mg/L NAA and 0.5mg/L biotin,which reached 2.01 cm.

关 键 词：油茶 优良无性系 组织培养 增殖体系 

分 类 号：S727.3[农业科学—林木遗传育种] S794.4[农业科学—林学]