带状疱疹患者淋巴母细胞在传代培养过程中染色体的稳定性分析  

The stability of the chromosomes during culture of lymphoblastoid cell lines from patients with herpes zoster

作　　者：莫小辉 杨连娟 潘会君 胡专 余茜 胡婷婷 吴飞 谭飞[1,3] 

机构地区：[1]上海市皮肤病医院中心实验室,上海200443 [2]上海市第一妇幼保健院产前诊断中心,上海200043 [3]安徽医科大学上海皮肤性病临床学院,上海200443

出　　处：《国际检验医学杂志》2015年第19期2798-2799,共2页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(81502738);上海市卫计委课题项目(20124y085);上海市公共卫生重点学科建设项目(12GWZX0902)

摘　　要：目的评价EB病毒转化的淋巴母细胞在传代培养过程中染色体的稳定性。方法采用EB病毒感染带状疱疹患者外周血中的B淋巴细胞,形成的淋巴母细胞(LCL)经过6个月的传代培养,通过核型分析比较淋巴母细胞染色体在培养前后是否存在差异。结果 4株染色体正常的LCL培养6个月后染色体无明显差异,1株染色体异常的LCL在传代培养过程中退化死亡,1株LCL培养6个月后染色体出现了亚二倍体和超二倍体。结论核型正常的LCL在传代培养过程中染色体具有较高的稳定性,选择核型正常的LCL有助于建立稳定的细胞系。Objective To evaluate the stability of the chromosomes during culture of lymphoblastoid cell lines （LCLs） from pa- tients with herpes zoster. Methods Established continuous cultures of LCLs for 6 months and analyzed the cells using conventional karyotyping. Results There were four LCLs with normal karyotyping had no change of chromosome structure during culturing, while one LCL with abnormal karyotyping showed chromosome aberrations and one LCL died. Conclusion It is feasible to main rain LCLs with a normal genomic structure during the LCL establishment processes.

关 键 词：EB病毒 淋巴母细胞系 核型分析 

分 类 号：R752.12[医药卫生—皮肤病学与性病学]