比较酶联免疫吸附法与间接免疫荧光法检测抗核抗体的价值  

Clinical significance of enzyme linked immunosorbent assay and indirect immunofluorescence for detection of antinuclear antibody

作　　者：王广杰[1] 陈洁[1] 李士军[1] 

机构地区：[1]大连医科大学附属第一医院检验科,辽宁大连116011

出　　处：《国际检验医学杂志》2015年第19期2814-2816,共3页International Journal of Laboratory Medicine

摘　　要：目的比较ELISA法和间接免疫荧光(IIF)法诊断自身免疫疾病的价值。方法系统性红斑狼疮(SLE)组33例,非SLE自身免疫疾病组59例,非自身免疫疾病组43例,同期该院体检中心健康体检者20例纳入健康对照组。采用2种方法检测4组研究对象的抗核抗体(ANA),并进行分析。结果 ELISA法检测SLE组、非SLE自身免疫疾病组中高效价的ANA分别为(2.621±1.700)、(2.248±1.781),IIF法分别为(2.715±0.730)、(2.544±0.59),但前者测出非自身免疫疾病中的ANA(1.034±1.050)低于后者ANA的效价(2.253±0.691)。结论 ELISA检测可提高ANA的检测效果。Objective To compare the value of ELISA and indirect immunofluorescence （IIF） method in diagnosis for autoim mune diseases. Methods A total of 33 patients in systemic lupus erythematosus（SLE）group,59 patients in other autoimmune dis eases group,43 patients in non-autoimmune disease group,20 people accepted physical examination in control group. The antinuclear antibody （ANA） in each group were detected by two methods and analyzed. Results The high titer ANA detected by EI.ISA and IIF in SLE group and other autoimmune diseases group were （2. 621±1. 700） ,（2. 248±1. 781） ;（2. 715±0. 730） ,（2. 544±0.59）. The titer ANA detected by ELISA in non-autoimmune disease group was （1. 034±1. 050）,which was lower than（2. 253±0. 691） detected by IIF. Conclusion ELSA might improve the detection effects of ANA antibody.

关 键 词：抗核抗体 酶联免疫吸附法 间接免疫荧光法 

分 类 号：R446.6[医药卫生—诊断学]