10-羟基喜树碱对类风湿关节炎患者成纤维样滑膜细胞表达血管内皮生长因子水平的影响  

Effect of 10-hydroxycamptotbecine on the expression of VEGF in rheumatoid arthritis synovial fibroblasts

作　　者：任丽丽[1] 王燕[1] 孙晓麟[2] 王少亭[1] 杨宏[1] 张丽娟[1] 张濛[1] 

机构地区：[1]郑州大学第五附属医院风湿免疫科,450002 [2]北京大学人民医院风湿免疫科

出　　处：《中华医学杂志》2015年第35期2834-2836,共3页National Medical Journal of China

基　　金：河南省教育厅重点科技攻关项目（14A320053）

摘　　要：目的 探讨10-羟基喜树碱（10-HCPT）对类风湿关节炎患者成纤维样滑膜细胞（RASF）表达血管内皮生长因子（VEGF）水平的影响.方法 体外分离培养RA患者成纤维样滑膜细胞,用不同剂量的10-HCPT（3.0×10-6 mol/L、3.0×10-5 mol/L）及甲氨蝶呤（MTX） （2.0×10-6 mol/L、2.0×10-5 mol/L）处理RASF 48 h后,不含药物的细胞作为对照组,用酶联免疫吸附方法（ELISA）检测细胞上清液中VEGF的表达水平.结果 10-HCPT高、低剂量组与对照组比较均差异有统计学意义（P＜0.05）,MTX高、低剂量与对照组比较均差异无统计学意义（P＞0.05）.10-HCPT高、低剂量组与MTX低剂量组比较差异有统计学意义（P＜0.05）;10-HCPT高剂量组与MTX高剂量组比较差异有统计学意义（P＜0.05）.结论 10-HCPT能抑制RASF表达VEGF水平,且其抑制作用较MTX更强.Objective To study the effect of 10-Hydroxycamptotbecine （10-HCPT） on the expression of vascular endothelial growth factor （VEGF） in rheumatoid arthritis synovial fibroblasts （RASFs）.Methods RASFs were isolated form synovial tissue of RA patients and cultured in vitro.10-HCPT（3.0 × 10-6 mol/L,3.0 × 10-5 mol/L） and Methotrexate （MTX） （2.0 × 10-6 mol/L,2.0 × 10-5 mol/L） with different concentrations were used to treat RASFs for 48 hours,and RASFs without 10-HCPT and MTX treatment were served as the control group.The level of VEGF in the supernatant of RASFs culture was measured by enzyme linked immunosorbent assay （ELISA）.Results Compared with control group,high and low dose 10-HCPT groups showed significant values on the effect of inhibiting the expression of VEGF in RASFs.Compared with low-dose MTX group,both high and low dose 10-HCPT groups showed significant difference （P 〈 0.05）;compared with high-dose MTX group,the high-dose 10-HCPT group had significant difference （P 〈 0.05）.Conclusion Compared with MTX,10-HCPT showed significant effect on inhibiting the expression of VEGF in RASFs.

关 键 词：10-羟基喜树碱 类风湿关节炎 血管内皮生长因子 成纤维样滑膜细胞 

分 类 号：R593.22[医药卫生—内科学]