薄层色谱法鉴定蓝狐胆汁及其牛磺熊去氧胆酸对四氯化碳肝损伤的保护作用  

Economy class animal blue fox bile for TLC identification and its protection against CCL_4 hepatic injury

作　　者：周博[1] 贾竞波[2] 李佳铭 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150050 [2]东北林业大学野生动物资源学院,哈尔滨150001 [3]哈尔滨市第五医院,150051

出　　处：《武警医学》2015年第8期783-786,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：黑龙江省教育厅科学技术研究项目(12531656)

摘　　要：目的确定蓝狐胆汁的药理作用。方法取30只昆明种小鼠,分为5组,每组6只。分别为:(1)空白组,非肝损伤处理,投喂蒸馏水,注射无伤害性的豆油;(2)模型组,肝损伤处理,不投喂任何药物,完全为四氯化碳(CCL4)急性肝损伤个体;(3)阳性组,肝损伤处理,投喂护肝片,0.4 g/kg;(4)高剂量组,肝损伤处理,投喂高剂量蓝狐胆汁粉,10 g/kg;(5)低剂量组,肝损伤处理,投喂低剂量的蓝狐胆汁粉,5 g/kg。首先用TLC法研究蓝狐胆汁成分中是否含有熊胆特有成分——牛磺熊去氧胆酸(UDCA),其次研究其胆汁对小鼠CCL4肝损伤是否有保护作用。结果对比蓝狐胆汁与熊胆汁,两者的相似度约50%,两者共四类物质中,有两类相同,蓝狐胆汁中含有UDCA成分。实验动物的肝脏被CCL4损伤之后,血清中的AST、ALT浓度明显增高,模型组与空白组对比,差异有统计学意义(P<0.05)。通过药理结果及病理切片,可以看到具有抑制CCL4,造成急性肝损伤小鼠AST、ALT浓度降低的作用。结论蓝狐胆汁对损伤的肝细胞有修复意义,对肝脏有明显的保护作用。Objective To determine the pharmacological effects of blue fox bile. Method Thirty Kunming mice were divided into 5 groups,each group of six.（ 1） For the blank group,without the liver damage treatment,was fed on distilled water,and harmless soybean oil was injected.（ 2） For the model group with liver damage,no drugs were given,were completely CCL4 acute liver injury individuals.（ 3） For positive group with liver damage were fed on liver-protecting,0. 4 / kg.（ 4） For the high dose group with liver damage was fed on high dose of blue fox bile powder,10 g / kg.（ 5） For the low dose group with liver damage was fed on the low dose of blue fox bile powder,5 g / kg. The first study was using TLC method,to determine whether the blue fox bile contained ursodeoxy cholic acid（ UDCA）. The second study was to determine whether the blue fox bile has a protective effect on CCL4 caused hepatic injury in mice. Results The similarity between blue fox bile and bear bile is about 50%,both with two kinds of material,the same in the four kinds of material. Blue fox UDCA component is contained in bile. After experimental animals were inflicted on injury of the liver by CCL4,AST and ALT in serum concentration significantly increased; the difference between the model group and the blank group was statistically significant. Through pharmacological results and pharmacological slices,it can be seen that CCL4 caused AST,ALT concentration effect acute liver injury can be inhibited in mice. Conclusions Blue fox bile can repair injury of liver cells and has obvious protective effects on the liver.

关 键 词：蓝狐胆汁 谷草转氨酶 谷丙转氨酶 四氯化碳肝损伤 

分 类 号：R961.1[医药卫生—药理学]