氨磷汀对小鼠肠型急性放射病的防护作用及其机制  

Protective effect and mechanism of amifostine on intestinal acute radiation sickness

作　　者：赵致维 崔宇[2] 王丽梅[2] 善亚君[2] 柳晓兰[2] 唐红卫[3] 从玉文[2] 

机构地区：[1]徐州医学院,221000 [2]解放军军事医学科学院放射研究所,北京100850 [3]武警总医院消化内科,北京100039

出　　处：《武警医学》2015年第8期797-800,804,共5页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家新药创制重大专项(2013ZX09J13102-02C);国家自然科学基金面上项目(81272997)

摘　　要：目的研究氨磷汀(amifostine,WR2721)对小鼠肠型急性放射病的防护作用及其机制。方法建立小鼠肠型急性放射病模型。C57BL/6J小鼠随机分为对照组和WR2721给药组,接受15 Gy60Co-γ全身照射,照后移植健康小鼠骨髓细胞,观察小鼠受照后30 d的存活率和平均生存时间,以及用隐窝细胞微克隆实验,研究照后3.5 d隐窝细胞数量及小肠黏膜情况。结果对照组小鼠均在10 d内死亡,WR2721给药组存活率提高到80%;对照组和WR2721给药组平均隐窝数分别为(6.1±0.7)个、(28.0±1.1)个,差异有统计学意义(P<0.0001);对照组和WR2721给药组绒毛高度分别为(51.48±2.04)μm、(73.29±3.24)μm,差异有统计学意义(P<0.0001)。结论 WR2721对肠型急性放射病小鼠有保护作用,通过提高隐窝干细胞增殖活性,促进黏膜的恢复,提高小鼠的存活率。Objective To study the radioprotection effect and mechanism of amifostine（ WR2721） on intestinal acute radiation sickness. Methods Based on the intestinal acute radiation sickness model,C57 BL /6J mice were randomly divided into control group and group WR2721. Bone marrow cells from healthy mice were transplanted after 15 Gy60Co-γ shielded body irradiation. Then the survival rate in 30 d after irradiation,and the number of proliferative crypt cells and the small intestine mucosa situation with microcloning survival assay of mouse crypt cells at 3. 5d post-irradiation were observed. Results The mice in control group died within 10 days,survival rate in WR2721 administrered group increased to 80%; in the control group and WR2721 drug group,the average number of crypts were 6. 1 ± 0. 7,28 ± 1. 1,the difference was statistically significant（ P〈0. 0001）; in control group and WR2721 administered group,villus heights were（ 51. 48 ± 2. 04） μm,（ 73. 29 ± 3. 24） μm,the difference was statistically significant（ P〈0. 0001）. Conclusions WR2721 has significantly radioprotective effect on intestinal acute radiation sickness by improving the survival rate of mice,promoting crypt cells regeneration and protecting the intestinal mucosa.

关 键 词：WR2721 防护 肠型急性放射病 机制 

分 类 号：R818[医药卫生—放射医学]