硒-汞双元素标记策略识别硒蛋白/多肽  

Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide

作　　者：徐明[1,2] 杨利民[1] 王秋泉[1] 

机构地区：[1]厦门大学化学化工学院化学系谱学分析与仪器教育部重点实验室,厦门361005 [2]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085

出　　处：《分析化学》2015年第9期1265-1271,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(Nos.2135006;21275120;21475108;21407169)~~

摘　　要：提出并发展了一种基于电感耦合等离子体质谱(ICP-MS)的双元素标签标记策略来选择性识别和检测硒蛋白/多肽,其中内源元素硒(Se)作为硒蛋白/多肽分子的识别元素,外源元素汞(Hg)作为硒蛋白/多肽和含硒蛋白/多肽分子的区分元素。通过对硒代半胱氨酸(SeCys)和谷胱甘肽过氧化酶1(GPx1)两种模型分子的研究,外源邻羧基苯硫甲基汞(CH3Hg-THI)动态解离的CH3Hg+能够选择性标记硒代半胱氨酸残基(SeCys)中硒醇基(-Se H),但不能标记含硒蛋白/多肽分子的硒代蛋氨酸残基(Se Met)中的—SeCH3,进而依据Se和Hg的ICP-MS信号识别和检测硒蛋白/多肽。本方法应用于富硒酵母水溶性提取液的分析,结果表明,提取液中的硒蛋白/多肽能够被有效识别和检测,验证了Se-Hg双元素标签标记策略的发展是ICP-MS识别和检测硒蛋白/多肽的一种可行且优越的途径。An endogenous element-label plus exogenous element-tag strategy was proposed for inductively coupled plasma mass spectrometry （ICP-MS） to screen and discriminate a family of uhratrace but biological important biomolecules. The feasibility of this novel idea has been demonstrated when setting seleno （SeCys） and Se-containing （SeMet） proteins （peptides） as an example. Se-label naturally occurring in the biomole- cules acts an identifier for picking them up out of large amount of various coexisting proteins （ peptides）, and CH3Hg-tag that can bind to SeCys but not SeMet fulfills the task of discriminating seleno and Se-containing ones based on the Se and Hg signals on ICP-MS. After confirmed using SeCys and GPxl, the Se-Hg dual- element labeling strategy together with ICP-MS was applied to screen and discriminate seleno and Se-contai- ning proteins （peptides） in the water-soluble extracts of Se-enriched yeast, and seven selenoproteins （pep- tides） were detected with both ~Hg and S2Se signals out of fifteen Se-containing species using RPLC/ICP- MS, providing valuable information for further identification using a high-resolution structure-selective mass spectrometer. This endogenous element-label plus exogenous element-tag dual-element approach implies that ICP-MS is not only able to quantify targeted proteins （peptides） but also helpful to recognize targeted proteins during a discovery-based proteomic study.

关 键 词：硒 汞 硒蛋白/多肽 电感耦合等离子体质谱 

分 类 号：O629.7[理学—有机化学]