粘质沙雷氏菌NJZT-1产灵菌红素培养条件的优化  

Optimization of culture medium composition and culture conditions of Serratia marcescens for enhancing the production of prodigiosin

作　　者：赵银娟[1] 杨韵[1] 吴小芹[1] 

机构地区：[1]南方现代林业协同创新中心,南京林业大学林学院,江苏省有害生物入侵预防与控制重点实验室,南京210037

出　　处：《林业科技开发》2015年第5期130-134,共5页China Forestry Science and Technology

基　　金：国家自然科学青年基金(31100448);博士点基金项目(2113204120004);江苏省博士后基金项目;南京林业大学科技创新基金

摘　　要：灵菌红素是一类具有多种生物活性的天然红色素的总称,主要由粘质沙雷氏菌产生,通常都有3个吡咯环组成的甲氧基吡咯骨架结构。灵菌红素具有抗肿瘤、免疫抑制、抗菌性、抗疟疾等多种生物学活性。通过单因素实验,分析了碳源、氮源、无机盐、表面活性剂、初始p H、培养温度等因素对粘质沙雷氏菌生长量及灵菌红素合成的影响。结果表明:粘质沙雷氏菌最佳产灵菌红素的培养条件为温度28℃,p H 7.0,培养基中碳源为淀粉,氮源为乙酸铵,无机盐为Fe Cl3,表面活性剂为二甲亚砜(DMSO),在此条件下粘质沙雷氏菌产灵菌红素条件为最佳。Prodigiosin（PG） is produced by a variety of microorganisms with many kinds of biological activities such as anti- tumor, immunosuppressive, antibacterial activities. They usually existed three pyrrole ring composed of methoxy radical pyrrole skeleton structure. In this paper, we analyed mass growth of Serrcttia marcescens and the production of prodigiosin u- sing the single factor experiments including the factors such as the carbon source, nitrogen source, inorganic salt, surfac- tant, initial pH and temperature. The results showed that optimal culture conditions for enhancing the production of prodigi- osin by S. marcescens were the following, temperature 28 ℃, pH 7.0, starch was culture medium carbon source, ammo- nium, acetate as nitrogen source, FeC13 as inorganic salt, dimethyl sulfoxide （DMSO） as surfactant.

关 键 词：灵菌红素 粘质沙雷氏菌 培养基条件优化 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]