槲皮素对实验性牙周炎大鼠牙周组织的保护作用  

Protective effects of quercetin on the periodontal tissue in rats with periodontitis

作　　者：葛超[1] 杨冬茹[1] 武明轩[1] 李春年[1] 

机构地区：[1]河北医科大学口腔医院口腔内科.河北省口腔医学重点实验室,石家庄030001

出　　处：《实用口腔医学杂志》2015年第5期619-622,共4页Journal of Practical Stomatology

摘　　要：目的:探讨槲皮素对实验性牙周炎大鼠牙周组织的保护作用。方法:选取体重300 g左右纯种雄性Wistar大鼠30只,随机分为3组(n=10)。对照组正常喂养;单纯结扎组行牙周(双侧上颌第一磨牙)结扎(直径0.2 mm正畸结扎丝);槲皮素干预组在牙周结扎后予以槲皮素50 mg/(kg·d)灌胃,1次/d。8周后处死全部大鼠。光镜观察牙周组织形态,免疫组织化学染色法检测牙周组织中肿瘤坏死因子-α(TNF-α)、环氧化酶-2(COX-2)的水平。结果:单纯结扎组结缔组织内可见炎症细胞浸润,结合上皮向根方增殖,牙周袋形成,牙槽骨吸收。槲皮素干预组可见牙周炎症及牙周袋较单纯结扎组轻。TNF-α、COX-2水平在单纯结扎组和槲皮素干预组均较对照组明显升高,但槲皮素干预组明显低于单纯结扎组(P<0.05)。结论:槲皮素对实验性牙周炎大鼠牙周组织具有保护作用。Objective：To investigate the protective effects of quercetin on the periodontal tissue in Wistar rats with periodontitis. Methods：30 Wistar rats were randomly divided into 3 groups（n =1 0）：Control group （group N）,ligation group（group L）and liga-tion ＋quercetin group（group LQ）.Bilateral first molars of the rats in group L and LQ were cervically ligated by an orthodontic wire with the diameter of 0.2 mm.The rats in group LQ were then treated with quercetin by gavage at 50 mg/（kg＆#183;d）after ligation.After 8 weeks,the rats were killed.Continuous slices of the periodontal tissue were made for HE staining and immunohistochemistry stai-ning.The morphological changes and the expression of TNF-αand COX-2 in the periodontal tissue were observed under light micro-scope.Results：The inflammatory cells and periodontal pocket were observed in group LQ were less than those in group L.The levels of TNF-α,COX-2 in group LQ were lower than that those in the group L（P 〈0.05）.Conclusion：Quercetin has a protective effect on the periodontal tissues of Wistar rats with periodontitis.

关 键 词：槲皮素 牙周炎 牙周组织 WISTAR 大鼠 

分 类 号：R285.5[医药卫生—中药学]