转基因Bt棉对根际土壤微生物群落的影响（英文）  

Effects of transgenic Bt cotton on rhizospheric soil microbial communities

作　　者：刘根林[1] 

机构地区：[1]江苏省林业科学研究院,江苏南京211153

出　　处：《江苏林业科技》2015年第4期28-34,40,共8页Journal of Jiangsu Forestry Science & Technology

摘　　要：根际箱中种植2个转基因Bt棉品系99BC-4,99BC-8及其非Bt受体泗棉3(SM3)后,于花期采集其根际土壤。BIOLOG检测显示,整个168 h的实验室培养期间,根际土壤微生物群落的每孔颜色平均变化率(AWCD)随着时间的推移呈现出差异性的S型曲线;AWCD值显示,GP2板低于GN2板和ECO板,意味着供试土壤内革兰氏阳性菌对碳源的利用能力较低。功能多样性分析表明,99BC-8和SM3根际土壤微生物群落的丰富度高、多样性强,集中了较多的主导微生物种,因而表现出高代谢能力和功能多样性。PCA得出,GN2板前2个主成分分别占总变量的35.59%和19.37%,GP2板前2个主成分分别占30.57%和18.89%。推理认为,Bt毒蛋白可能刺激了某些微生物利用优势碳源以促其生长,主导着土壤微生物的反应,虽然在2个转基因Bt棉品系根际土壤中其表现不一致。After cultivation of 2 transgenic Bt cotton lines （99BC-4, 99BC-8） and their non-Bt recipient Simian3 （SM3） in rhizoboxes, the rhizospheric soils during blooming period were sampled. Biolog characterization revealed that during the whole laboratory incubation for 168 h, AWCDs of the rhizospheric soil microbial communities exhibited differential sigmoi- dais of C substrate utilization with incubation time, and the soils showed lower AWCDs in GP2 plate than in GN2 plate and EcoPlate, meaning that the G ＋ bacteria in the soils possessed a weaker C source metabolic ability. Functional diversity a- nalysis indicated that the rhizospheric soils of 99BC-8 and SM3 had a larger richness and diversity of, and centered more dominant species in the microbial communities, reflecting a high metabolic potential and functional diversity. Principal Component Analysis （PCA） identified that the first 2 PCs accounted respectively for 35.59%, 19.37% in GN2 plate, and 30.57% , 18.89% in GP2 plate of total variation. It can be deduced that Bt endotoxin likely stimulated the growth of some specific microbes by using the preferential C sources, which likely dominated the soil microbial responses, though inconsis- tent in 2 transzenic Bt lines.

关 键 词：转基因BT棉 Biolog微生物鉴定系统 土壤 微生物群落 主成分分析 

分 类 号：S154.36[农业科学—土壤学]