烟草环斑病毒抗体及DAS-ELISA试剂盒的研制  

Preparation of Antibody against Tobacco ringspot virus and DAS-ELISA Kit

作　　者：李桂芬[1] 魏梅生[1] 马洁[1] 张永江[1] 

机构地区：[1]中国检验检疫科学研究院,北京100121

出　　处：《检验检疫学刊》2015年第4期1-4,共4页Journal of Inspection and Quarantine

基　　金：质检公益性行业科研专项(201310071)

摘　　要：将纯化的烟草环斑病毒免疫家兔获得多克隆抗体;免疫BALB/c小鼠,用杂交瘤细胞技术获得1株分泌烟草环斑病毒单克隆抗体的杂交瘤细胞株并制备腹水抗体。以戊二醛为交联剂,采用两步法将烟草环斑病毒单克隆抗体用碱性磷酸酯酶标记,研制了烟草环斑病毒DAS-ELISA试剂盒。The purified virus of TRSV was used to immunize rabbit and polyclonal antibody was produced. One hybridoma cell line secreting monoclonal antibody against TRSV was produced by hybridoma technology. Alkaline phosphatase was conjugated with the monoclonal antibody by bifunctional linker glutaraldehyde,DAS-ELISA kit were produced.

关 键 词：烟草环斑病毒 多克隆抗体 单克隆抗体 DAS-ELISA试剂盒 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]