ELISA质控血清配制方法的研究  

Preparation of External Quality Control Serum for ELISA Test

作　　者：刘建礼[1] 王飞[1] 徐惠芳[1] 魏海燕[1] 张伟[1] 刘来福[1] 

机构地区：[1]北京出入境检验检疫局,北京100026

出　　处：《检验检疫学刊》2015年第4期61-63,60,共4页Journal of Inspection and Quarantine

基　　金：国家质检总局科技计划项目(2013IK306)

摘　　要：[目的]探讨HIV ELISA检测质控血清配制的关键影响因素及方法。[方法]选择HIV阳性血清以阴性血清进行系列稀释,直至获得S/CO=2-3之间的质控血清,以PBS、牛血清白蛋白、二甲基亚砜(DMSO)、甘油等组成不同稀释液,探讨各种稀释液与阴性血清稀释效果的不同。[结果]HIV强阳性血清在稀释初期,S/CO值下降缓慢,在S/CO值下降至15以下时,可以用倍比稀释的方法获得相应靶值的质控血清;PBS、PBS+10%BSA、阴性血清、阴性血清+10%DMSO、阴性血清+10%甘油作为稀释液,获得的质控血清S/CO值差别不明显。[结论]强阳性血清须先采用高倍数稀释,然后再进行倍比稀释寻找S/CO在2-3的最终稀释度;PBS加BSA可以替代阴性血清作为稀释液。Objective] To explore the crucial factors in the preparation of external quality control serum for HIV ELISA test. [Methods] HIV positive serum was confirmed by ELISA and Western blotting,then it was diluted by HIV negative normal serum until the S/CO value reached 2 to 3 in ELISA test. Four different dilutions were also used and compared with normal serum by S/CO value. [Results] Strong positive serum selected showed high S/CO value of 21 to 30. The value did not decrease notably until it reached the value blow 15,after that control serum of OD value 2 to 3 could be made by double？ratio？ dilution. PBS,PBS＋BSA did not show difference compared with normal serum when they were used as dilution. [Conclusion] Strong positive HIV serum often has high value of S/CO,high ratio dilution should be made before the value decrease notably,then control serum of 2-3 value could be made by serial dilution. PBS＋BSA could be a substitute of normal serum as dilution.

关 键 词：质控血清 配制方法 稀释液 

分 类 号：R446.61[医药卫生—诊断学]