化滞柔肝颗粒对酒精联合脂多糖诱导的酒精性肝炎小鼠的保护作用  

Protective effects of Huazhirougan granule on liver tissue injuries of mice with alcohol and lipopolysaccharide-induced alcoholic hepatitis

作　　者：关永霞 李晓梅 张永霞 李晓丽 王平平 张贵民 

机构地区：[1]鲁南制药集团股份有限公司新药药理中心/中药制药共性技术国家重点实验室药效学研究中心,山东省临沂市费县273400

出　　处：《实用肝脏病杂志》2015年第5期530-533,共4页Journal of Practical Hepatology

基　　金：国家科技部973项目(编号:2012CB724001)

摘　　要：目的观察化滞柔肝颗粒对酒精联合脂多糖诱导的酒精性肝炎小鼠的保护作用。方法将80只ICR小鼠随机分成正常组、模型组、小、中、大剂量化滞柔肝组和护肝片处理组,通过灌胃给予56%北京红星二锅头(12ml·kg^(-1).d^(-1))和脂多糖(5 mg·kg^(-1),2次/w)腹腔注射,建立酒精性肝炎小鼠模型,同时给予相应的药物灌胃,连续10w。在末次给药后,解剖动物,取血清和肝组织,进行相应的检查。结果在实验10 w末,模型组肝组织肝细胞水肿,胞浆疏松,其肝质量指数、血清ALT和AST水平分别为(5.77±0.67)%、(82.22±6.20)U/L和(93.43±17.30)U/L,均显著高于正常组[分别为(4.44±0.42)%、(35.83±3.84)U/L和(66.43±5.14)U/L,P<0.05];大剂量化滞柔肝组肝质量指数为(5.24±0.36)%,显著低于模型组[(5.77±0.67)%,P<0.05],小、中、大剂量化滞柔肝颗粒组和护肝片组ALT和AST水平均显著低于模型组(P<0.001);小、中、大剂量化滞柔肝颗粒组和护肝片组的CK分别为(118.93±10.15)U/L、(102.33±8.07)U/L、(119.45±19.26)U/L和(104.00±8.15)U/L,均显著低于模型组[(227.50±50.10)U/L,P<0.001];小、中、大剂量化滞柔肝组和护肝片组血清TBIL水平均显著低于模型组(P<0.01);各试验组之间血脂水平均无明显变化。结论化滞柔肝颗粒对酒精联合脂多糖诱导的酒精性肝炎小鼠有保护作用,为化滞柔肝颗粒将来用于临床治疗酒精性脂肪肝提供了试验支持。Objective To study the protective effects of Huazhirougan granule on liver tissue injuries of mice with alcohol and lipopolysaccharide-induced alcoholic hepatitis. Methods Eighty mice were randomly divid-ed into control,model,and low,middle and high dose of Huazhirougan granule,and Hugan tablet group. Except in the control group,the mice in other five groups had 56% alcohol by intragastric administration once daily,and in-traperitoneal administration of lipopolysaccharide twice a week,and then intragastricly administered corresponding drugs in the afternoon for 10 weeks. The serum levels of ALT,AST,CK,γ-GT,ALP,TBIL,TG,TC,HDL-C and LDL-C were measured. The liver tissues were fixed by formaldehyde,routine paraffin section,and hematoxylin-eosin staining. Results The liver mass index,serum ALT and AST levels in the model group increased signifi-cantly [（5.77±0.67）%,（82.22±6.20） U/L and （93.43±17.30） U/L] vs. [（4.44±0.42）%,（35.83±3.84） U/L and （66.43±5.14） U/L,P〈0.05] as compared with in control group; the serum levels of transaminase in the low,mid-dle,and high dose of Huazhirougan granule group,and Hugan tablet group decreased significantly as compared to those in model group （P〈0.001）;The serum CK levels in the low,middle,and high dose of Huazhirougan granule group,and Hugan tablet group were （118.93±10.15） U/L,（102.33±8.07） U/L,（119.45±19.26） U/L,（104.00±8.15） U/L,significantly decreased as compared with in model group [（227.50±50.10） U/L,P〈0.001];The serum bilirubin levels also decreased significantly as compared with in model group （P〈0.01）. Conclusion Huazhirougan granule can protect the alcoholic liver injuries in mice with alcohol and lipopolysaccharide- induced alcoholic hepatitis.

关 键 词：酒精性肝炎 化滞柔肝颗粒 脂多糖 小鼠 

分 类 号：R285[医药卫生—中药学]